Serological and molecular evidence of hepadnavirus infection in swine.
Ann Agric Environ Med
; 22(1): 11-6, 2015.
Article
em En
| MEDLINE
| ID: mdl-25780820
ABSTRACT
INTRODUCTION AND OBJECTIVE:
Recently, investigations in a swine herd identified evidence of the existence of a novel member of the Hepadnavirus family endemic in swine. The aim of this study was to investigate the serological and molecular markers of Hepadnavirus circulation in Brazilian domestic swine and wild boar herds, and to evaluate the identity with HBV and other Hepadnaviruses reported previously. MATERIALS ANDMETHODS:
For the study, 376 swine were screened for hepatitis B virus serological markers. Analyses were performed in serum samples using commercial enzyme-linked immunosorbent assay (ELISA) kits (DiaSorin®) for anti-HBc, HBsAg and anti-HBs. Reactive and undetermined swine serum samples were selected to perform DNA viral extraction (QIAamp DNA Mini Kit, Qiagen®), partial genome amplification and genome sequencing.RESULTS:
From 376 swine samples analysed, 28 (7.45%) were reactive to anti-HBc, 3 (0.80%) to HBsAg and 6 (1.6%) to anti-HBs. Besides, more 17 (4.52%) swine samples analyzed were classified in the grey zone of the EIA test to anti-HBc and 2 (0.53%) to HBsAg. From 49 samples molecularly analyzed after serological trial, 4 samples showed a positive result for the qualitative PCR for Hepadnavirus. Phylogenetic reconstruction using partial genome sequencing (360 bp) of 3 samples showed similarity with HBV with 90.8-96.3% of identity.CONCLUSIONS:
Serological and molecular data showed evidence of the circulation of a virus similar to hepatitis B virus in swine.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Doenças dos Suínos
/
Vírus da Hepatite B
/
Hepatite B
Tipo de estudo:
Qualitative_research
Limite:
Animals
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Ann Agric Environ Med
Assunto da revista:
SAUDE AMBIENTAL
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Brasil