Genotoxicity of tungsten carbide-cobalt (WC-Co) nanoparticles in vitro: mechanisms-of-action studies.
Mutat Res Genet Toxicol Environ Mutagen
; 779: 15-22, 2015 Feb.
Article
em En
| MEDLINE
| ID: mdl-25813722
ABSTRACT
We showed previously that tungsten carbide-cobalt (WC-Co) nanoparticles (NP) can be used as a nanoparticulate positive control in some in vitro mammalian genotoxicity assays. Here, we investigate the mechanisms of action involved in WC-Co NP genotoxicity in L5178Y mouse lymphoma cells and primary human lymphocytes, in vitro. Data from the micronucleus assay coupled with centromere staining and from the chromosome-aberration assay show the involvement of both clastogenic and aneugenic events. Experiments with the formamidopyrimidine DNA glycosylase (FPG)-modified comet assay showed a slight (non-significant) increase in FPG-sensitive sites in the L5178Y mouse lymphoma cells but not in the human lymphocytes. Electron paramagnetic resonance spin-trapping results showed the presence of hydroxyl radicals (â¢OH) in WC-Co NP suspensions, with or without cells, but with time-dependent production in the presence of cells. However, a significant difference in â¢OH production was observed between human lymphocytes from two different donors. Using H2O2, we showed that WC-Co NP can participate in Fenton-like reactions. Thus, â¢OH might be produced either via intrinsic generation by WC-Co NP or through a Fenton-like reaction in the presence of cells.
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Texto completo:
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Bases de dados:
MEDLINE
Assunto principal:
Dano ao DNA
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Cobalto
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Compostos de Tungstênio
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Nanopartículas
Limite:
Animals
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Humans
Idioma:
En
Revista:
Mutat Res Genet Toxicol Environ Mutagen
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
França