Feeding soy protein isolate and n-3 PUFA affects polycystic liver disease progression in a PCK rat model of autosomal polycystic kidney disease.
J Pediatr Gastroenterol Nutr
; 60(4): 467-73, 2015 Apr.
Article
em En
| MEDLINE
| ID: mdl-25822773
ABSTRACT
OBJECTIVE:
In polycystic liver disease (PCLD), multiple cysts cause liver enlargement, structural damage, and loss of function. Soy protein and dietary ω-3 polyunsaturated fatty acids (n-3 PUFAs) have been found to decrease cyst proliferation and inflammation in polycystic kidney disease. Therefore, the aim of the study was to investigate whether soy protein and n-3 PUFA supplementation attenuates PCLD.METHODS:
Young (age 28 days) female PCK rats were fed (nâ=â12 per group) either caseinâ+âcorn oil (caseinâ+âCO), caseinâ+âsoybean oil (caseinâ+âSO), soy protein isolateâ+âsoybean oil (SPIâ+âSO), or SPIâ+â11âsoybean/salmon oil blend (SPIâ+âSB) diet for 12 weeks. Liver histology, gene expression by real-time quantitative polymerase chain reaction, and serum markers of liver injury were determined.RESULTS:
Diet had no effect on PCLD progression as indicated by no significant differences in liver weight and hepatic proliferation gene expression between diet groups. PCK rats fed SPIâ+âSB diet, however, had the greatest (Pâ<â0.05) histological evidence of hepatic cyst obstruction, portal inflammation, steatosis, and upregulation (Pâ=â0.03) of fibrosis-related genes. Rats fed SPIâ+âSB diet also had the lowest (Pâ<â0.001) serum cholesterol and higher (Pâ<â0.05) serum alkaline phosphatase and bilirubin concentrations.CONCLUSIONS:
Feeding young female PCK rats SPI and n-3 PUFA failed to attenuate PCLD progression. Furthermore, feeding SPIâ+âSB diet resulted in complications of hepatic steatosis attributable to cysts obstruction of bile duct and hepatic vein. Based on the results, it was concluded that diet intervention alone was not effective at attenuating PCLD associated with autosomal recessive polycystic kidney disease.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Ácidos Graxos Ômega-3
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Rim Policístico Autossômico Recessivo
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Proteínas de Soja
/
Suplementos Nutricionais
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Cistos
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Fígado
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Hepatopatias
Tipo de estudo:
Etiology_studies
Limite:
Animals
Idioma:
En
Revista:
J Pediatr Gastroenterol Nutr
Ano de publicação:
2015
Tipo de documento:
Article