Your browser doesn't support javascript.
loading
DprE1 Is a Vulnerable Tuberculosis Drug Target Due to Its Cell Wall Localization.
Brecik, Miroslav; Centárová, Ivana; Mukherjee, Raju; Kolly, Gaëlle S; Huszár, Stanislav; Bobovská, Adela; Kilacsková, Emöke; Mokosová, Veronika; Svetlíková, Zuzana; Sarkan, Michal; Neres, João; Korduláková, Jana; Cole, Stewart T; Mikusová, Katarína.
Afiliação
  • Brecik M; †Department of Biochemistry, Faculty of Natural Sciences, Comenius University in Bratislava, 842 15 Bratislava, Slovakia.
  • Centárová I; †Department of Biochemistry, Faculty of Natural Sciences, Comenius University in Bratislava, 842 15 Bratislava, Slovakia.
  • Mukherjee R; ‡Global Health Institute, Ecole Polytechnique Fédérale de Lausanne, 1015 Lausanne, Switzerland.
  • Kolly GS; ‡Global Health Institute, Ecole Polytechnique Fédérale de Lausanne, 1015 Lausanne, Switzerland.
  • Huszár S; †Department of Biochemistry, Faculty of Natural Sciences, Comenius University in Bratislava, 842 15 Bratislava, Slovakia.
  • Bobovská A; †Department of Biochemistry, Faculty of Natural Sciences, Comenius University in Bratislava, 842 15 Bratislava, Slovakia.
  • Kilacsková E; †Department of Biochemistry, Faculty of Natural Sciences, Comenius University in Bratislava, 842 15 Bratislava, Slovakia.
  • Mokosová V; †Department of Biochemistry, Faculty of Natural Sciences, Comenius University in Bratislava, 842 15 Bratislava, Slovakia.
  • Svetlíková Z; †Department of Biochemistry, Faculty of Natural Sciences, Comenius University in Bratislava, 842 15 Bratislava, Slovakia.
  • Sarkan M; †Department of Biochemistry, Faculty of Natural Sciences, Comenius University in Bratislava, 842 15 Bratislava, Slovakia.
  • Neres J; ‡Global Health Institute, Ecole Polytechnique Fédérale de Lausanne, 1015 Lausanne, Switzerland.
  • Korduláková J; †Department of Biochemistry, Faculty of Natural Sciences, Comenius University in Bratislava, 842 15 Bratislava, Slovakia.
  • Cole ST; ‡Global Health Institute, Ecole Polytechnique Fédérale de Lausanne, 1015 Lausanne, Switzerland.
  • Mikusová K; †Department of Biochemistry, Faculty of Natural Sciences, Comenius University in Bratislava, 842 15 Bratislava, Slovakia.
ACS Chem Biol ; 10(7): 1631-6, 2015 Jul 17.
Article em En | MEDLINE | ID: mdl-25906160
ABSTRACT
The flavo-enzyme DprE1 catalyzes a key epimerization step in the decaprenyl-phosphoryl d-arabinose (DPA) pathway, which is essential for mycobacterial cell wall biogenesis and targeted by several new tuberculosis drug candidates. Here, using differential radiolabeling with DPA precursors and high-resolution fluorescence microscopy, we disclose the unexpected extracytoplasmic localization of DprE1 and periplasmic synthesis of DPA. Collectively, this explains the vulnerability of DprE1 and the remarkable potency of the best inhibitors.
Assuntos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Tuberculose / Parede Celular / Oxirredutases do Álcool / Mycobacterium tuberculosis / Antituberculosos Limite: Humans Idioma: En Revista: ACS Chem Biol Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Eslováquia
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Tuberculose / Parede Celular / Oxirredutases do Álcool / Mycobacterium tuberculosis / Antituberculosos Limite: Humans Idioma: En Revista: ACS Chem Biol Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Eslováquia