Human definitive haemogenic endothelium and arterial vascular endothelium represent distinct lineages.
Nat Cell Biol
; 17(5): 580-91, 2015 May.
Article
em En
| MEDLINE
| ID: mdl-25915127
ABSTRACT
The generation of haematopoietic stem cells (HSCs) from human pluripotent stem cells (hPSCs) will depend on the accurate recapitulation of embryonic haematopoiesis. In the early embryo, HSCs develop from the haemogenic endothelium (HE) and are specified in a Notch-dependent manner through a process named endothelial-to-haematopoietic transition (EHT). As HE is associated with arteries, it is assumed that it represents a subpopulation of arterial vascular endothelium (VE). Here we demonstrate at a clonal level that hPSC-derived HE and VE represent separate lineages. HE is restricted to the CD34(+)CD73(-)CD184(-) fraction of day 8 embryoid bodies and it undergoes a NOTCH-dependent EHT to generate RUNX1C(+) cells with multilineage potential. Arterial and venous VE progenitors, in contrast, segregate to the CD34(+)CD73(med)CD184(+) and CD34(+)CD73(hi)CD184(-) fractions, respectively. Together, these findings identify HE as distinct from VE and provide a platform for defining the signalling pathways that regulate their specification to functional HSCs.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Artérias
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Células-Tronco Hematopoéticas
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Diferenciação Celular
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Linhagem da Célula
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Células-Tronco Multipotentes
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Células-Tronco Pluripotentes
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Células Progenitoras Endoteliais
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Nat Cell Biol
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Canadá