The spatial profiles and metabolic capabilities of microbial populations impact the growth of antibiotic-resistant mutants.
J R Soc Interface
; 12(107)2015 Jun 06.
Article
em En
| MEDLINE
| ID: mdl-25972434
ABSTRACT
Antibiotic resistance adversely affects clinical and public health on a global scale. Using the opportunistic human pathogen Pseudomonas aeruginosa, we show that increasing the number density of bacteria, on agar containing aminoglycoside antibiotics, can non-monotonically impact the survival of antibiotic-resistant mutants. Notably, at high cell densities, mutant survival is inhibited. A wide range of bacterial species can inhibit antibiotic-resistant mutants. Inhibition results from the metabolic breakdown of amino acids, which results in alkaline by-products. The consequent increase in pH acts in conjunction with aminoglycosides to mediate inhibition. Our work raises the possibility that the manipulation of microbial population structure and nutrient environment in conjunction with existing antibiotics could provide therapeutic approaches to combat antibiotic resistance.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Pseudomonas aeruginosa
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Farmacorresistência Bacteriana
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Viabilidade Microbiana
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Antibacterianos
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Modelos Biológicos
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Mutação
Limite:
Humans
Idioma:
En
Revista:
J R Soc Interface
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Estados Unidos