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Soluble Epoxide Hydrolase in Hydrocephalus, Cerebral Edema, and Vascular Inflammation After Subarachnoid Hemorrhage.
Siler, Dominic A; Berlow, Yosef A; Kukino, Ayaka; Davis, Catherine M; Nelson, Jonathan W; Grafe, Marjorie R; Ono, Hirohisa; Cetas, Justin S; Pike, Martin; Alkayed, Nabil J.
Afiliação
  • Siler DA; From the Department of Anesthesiology and Perioperative Medicine, The Knight Cardiovascular Institute (D.A.S., C.M.D., J.W.N., M.R.G., N.J.A.), Department of Neurological Surgery (D.A.S., J.S.C.), Advanced Imaging Research Center (Y.A.B., A.K., M.P.), Oregon Health and Science University, Portland;
  • Berlow YA; From the Department of Anesthesiology and Perioperative Medicine, The Knight Cardiovascular Institute (D.A.S., C.M.D., J.W.N., M.R.G., N.J.A.), Department of Neurological Surgery (D.A.S., J.S.C.), Advanced Imaging Research Center (Y.A.B., A.K., M.P.), Oregon Health and Science University, Portland;
  • Kukino A; From the Department of Anesthesiology and Perioperative Medicine, The Knight Cardiovascular Institute (D.A.S., C.M.D., J.W.N., M.R.G., N.J.A.), Department of Neurological Surgery (D.A.S., J.S.C.), Advanced Imaging Research Center (Y.A.B., A.K., M.P.), Oregon Health and Science University, Portland;
  • Davis CM; From the Department of Anesthesiology and Perioperative Medicine, The Knight Cardiovascular Institute (D.A.S., C.M.D., J.W.N., M.R.G., N.J.A.), Department of Neurological Surgery (D.A.S., J.S.C.), Advanced Imaging Research Center (Y.A.B., A.K., M.P.), Oregon Health and Science University, Portland;
  • Nelson JW; From the Department of Anesthesiology and Perioperative Medicine, The Knight Cardiovascular Institute (D.A.S., C.M.D., J.W.N., M.R.G., N.J.A.), Department of Neurological Surgery (D.A.S., J.S.C.), Advanced Imaging Research Center (Y.A.B., A.K., M.P.), Oregon Health and Science University, Portland;
  • Grafe MR; From the Department of Anesthesiology and Perioperative Medicine, The Knight Cardiovascular Institute (D.A.S., C.M.D., J.W.N., M.R.G., N.J.A.), Department of Neurological Surgery (D.A.S., J.S.C.), Advanced Imaging Research Center (Y.A.B., A.K., M.P.), Oregon Health and Science University, Portland;
  • Ono H; From the Department of Anesthesiology and Perioperative Medicine, The Knight Cardiovascular Institute (D.A.S., C.M.D., J.W.N., M.R.G., N.J.A.), Department of Neurological Surgery (D.A.S., J.S.C.), Advanced Imaging Research Center (Y.A.B., A.K., M.P.), Oregon Health and Science University, Portland;
  • Cetas JS; From the Department of Anesthesiology and Perioperative Medicine, The Knight Cardiovascular Institute (D.A.S., C.M.D., J.W.N., M.R.G., N.J.A.), Department of Neurological Surgery (D.A.S., J.S.C.), Advanced Imaging Research Center (Y.A.B., A.K., M.P.), Oregon Health and Science University, Portland;
  • Pike M; From the Department of Anesthesiology and Perioperative Medicine, The Knight Cardiovascular Institute (D.A.S., C.M.D., J.W.N., M.R.G., N.J.A.), Department of Neurological Surgery (D.A.S., J.S.C.), Advanced Imaging Research Center (Y.A.B., A.K., M.P.), Oregon Health and Science University, Portland;
  • Alkayed NJ; From the Department of Anesthesiology and Perioperative Medicine, The Knight Cardiovascular Institute (D.A.S., C.M.D., J.W.N., M.R.G., N.J.A.), Department of Neurological Surgery (D.A.S., J.S.C.), Advanced Imaging Research Center (Y.A.B., A.K., M.P.), Oregon Health and Science University, Portland;
Stroke ; 46(7): 1916-22, 2015 Jul.
Article em En | MEDLINE | ID: mdl-25991416
ABSTRACT
BACKGROUND AND

PURPOSE:

Acute communicating hydrocephalus and cerebral edema are common and serious complications of subarachnoid hemorrhage (SAH), whose causes are poorly understood. Using a mouse model of SAH, we determined whether soluble epoxide hydrolase (sEH) gene deletion protects against SAH-induced hydrocephalus and edema by increasing levels of vasoprotective eicosanoids and suppressing vascular inflammation.

METHODS:

SAH was induced via endovascular puncture in wild-type and sEH knockout mice. Hydrocephalus and tissue edema were assessed by T2-weighted magnetic resonance imaging. Endothelial activation was assessed in vivo using T2*-weighted magnetic resonance imaging after intravenous administration of iron oxide particles linked to anti-vascular cell adhesion molecule-1 antibody 24 hours after SAH. Behavioral outcome was assessed at 96 hours after SAH with the open field and accelerated rotarod tests.

RESULTS:

SAH induced an acute sustained communicating hydrocephalus within 6 hours of endovascular puncture in both wild-type and sEH knockout mice. This was followed by tissue edema, which peaked at 24 hours after SAH and was limited to white matter fiber tracts. sEH knockout mice had reduced edema, less vascular cell adhesion molecule-1 uptake, and improved outcome compared with wild-type mice.

CONCLUSIONS:

Genetic deletion of sEH reduces vascular inflammation and edema and improves outcome after SAH. sEH inhibition may serve as a novel therapy for SAH.
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Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Hemorragia Subaracnóidea / Vasculite / Edema Encefálico / Epóxido Hidrolases Limite: Animals Idioma: En Revista: Stroke Ano de publicação: 2015 Tipo de documento: Article

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Hemorragia Subaracnóidea / Vasculite / Edema Encefálico / Epóxido Hidrolases Limite: Animals Idioma: En Revista: Stroke Ano de publicação: 2015 Tipo de documento: Article