DNA Demethylation Dynamics in the Human Prenatal Germline.
Cell
; 161(6): 1425-36, 2015 Jun 04.
Article
em En
| MEDLINE
| ID: mdl-26004067
ABSTRACT
Global DNA demethylation in humans is a fundamental process that occurs in pre-implantation embryos and reversion to naive ground state pluripotent stem cells (PSCs). However, the extent of DNA methylation reprogramming in human germline cells is unknown. Here, we performed whole-genome bisulfite sequencing (WGBS) and RNA-sequencing (RNA-seq) of human prenatal germline cells from 53 to 137 days of development. We discovered that the transcriptome and methylome of human germline is distinct from both human PSCs and the inner cell mass (ICM) of human blastocysts. Using this resource to monitor the outcome of global DNA demethylation with reversion of primed PSCs to the naive ground state, we uncovered hotspots of ultralow methylation at transposons that are protected from demethylation in the germline and ICM. Taken together, the human germline serves as a valuable in vivo tool for monitoring the epigenome of cells that have emerged from a global DNA demethylation event.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Blastocisto
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Metilação de DNA
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Embrião de Mamíferos
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Células Germinativas
Limite:
Female
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Humans
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Male
Idioma:
En
Revista:
Cell
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Estados Unidos