Urinary myoglobin quantification by high-performance liquid chromatography: An alternative measurement for exercise-induced muscle damage.
Anal Biochem
; 491: 37-42, 2015 Dec 15.
Article
em En
| MEDLINE
| ID: mdl-26363103
ABSTRACT
This study investigated a means of quantifying urinary myoglobin using a novel reverse-phase high-performance liquid chromatography (RP-HPLC) method that is an alternative measure of exercise-induced muscle damage. It also investigated the effect of storage and alkalization on urinary myoglobin stability issues. An RP-HPLC method was validated by precision and repeatability experiments. Myoglobin stability was determined through spiked urine samples stored at various temperatures over an 8-week period using alkalization and dilution in a pH 7.0 buffer. The method was validated with urine collected from mixed martial arts fighters during a competition and training session. The method produced linearity from 5 to 1000 µg/ml (R(2) = 0.997), intra- and inter-assay coefficients of variation from 0.32 to 2.94%, and a lower detection limit of 0.2 µg/ml in the final dilution and 2 µg/ml in the original urine sample. Recovery ranged from 96.4 to 102.5%, myoglobin remained stable at 4 °C when diluted in a pH 7.0 buffer after 20 h, and a significant increase (P < 0.01) and an identifiable peak were observed following a mixed martial arts contest and training session. Storage length and conditions had significant effects (P < 0.05) on stability. The method's simplicity and noninvasive nature means it can be used as an alternative muscle damage assay following exercise and trauma.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Cromatografia Líquida de Alta Pressão
/
Urinálise
/
Músculo Esquelético
/
Mioglobina
Limite:
Adult
/
Humans
Idioma:
En
Revista:
Anal Biochem
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Nova Zelândia