A high-resolution imaging approach to investigate chromatin architecture in complex tissues.
Cell
; 163(1): 246-55, 2015 Sep 24.
Article
em En
| MEDLINE
| ID: mdl-26406379
ABSTRACT
We present ChromATin, a quantitative high-resolution imaging approach for investigating chromatin organization in complex tissues. This method combines analysis of epigenetic modifications by immunostaining, localization of specific DNA sequences by FISH, and high-resolution segregation of nuclear compartments using array tomography (AT) imaging. We then apply this approach to examine how the genome is organized in the mammalian brain using female Rett syndrome mice, which are a mosaic of normal and Mecp2-null cells. Side-by-side comparisons within the same field reveal distinct heterochromatin territories in wild-type neurons that are altered in Mecp2-null nuclei. Mutant neurons exhibit increased chromatin compaction and a striking redistribution of the H4K20me3 histone modification into pericentromeric heterochromatin, a territory occupied normally by MeCP2. These events are not observed in every neuronal cell type, highlighting ChromATin as a powerful in situ method for examining cell-type-specific differences in chromatin architecture in complex tissues.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Processamento de Imagem Assistida por Computador
/
Encéfalo
/
Cromatina
/
Tomografia
/
Síndrome de Rett
Limite:
Animals
Idioma:
En
Revista:
Cell
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Estados Unidos