Analysis of Salmonella PhoP/PhoQ regulation by dimethyl-SRM-based quantitative proteomics.
Biochim Biophys Acta
; 1864(1): 20-8, 2016 Jan.
Article
em En
| MEDLINE
| ID: mdl-26472331
ABSTRACT
SRM (selected reaction monitoring), a tandem mass spectrometry-based method characterized by high repeatability and accuracy, is an effective tool for the quantification of predetermined proteins. In this study, we built a time-scheduled dimethyl-SRM method that can provide the precise relative quantification of 92 proteins in one run. By applying this method to the Salmonella PhoP/PhoQ two-component system, we found that the expression of selected PhoP/PhoQ-activated proteins in response to Mg(2+) concentrations could be divided into two distinct patterns. For the time-course SRM experiment, we found that the dynamics of the selected PhoP/PhoQ-activated proteins could be divided into three distinct patterns, providing a new clue regarding PhoP/PhoQ activation and regulation. Moreover, the results for iron homeostasis proteins in response to Mg(2+) concentrations revealed that the PhoP/PhoQ two-component system may serve as a repressor for iron uptake proteins. And ribosomal protein levels clearly showed a response to different Mg(2+) concentrations and to time.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Salmonella
/
Proteínas de Bactérias
/
Biologia Computacional
/
Proteômica
/
Espectrometria de Massas em Tandem
Idioma:
En
Revista:
Biochim Biophys Acta
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
China