A Hybrid Drug Limits Resistance by Evading the Action of the Multiple Antibiotic Resistance Pathway.
Mol Biol Evol
; 33(2): 492-500, 2016 Feb.
Article
em En
| MEDLINE
| ID: mdl-26538141
ABSTRACT
Hybrid drugs are a promising strategy to address the growing problem of drug resistance, but the mechanism by which they modulate the evolution of resistance is poorly understood. Integrating high-throughput resistance measurements and genomic sequencing, we compared Escherichia coli populations evolved in a hybrid antibiotic that links ciprofloxacin and neomycin B with populations evolved in combinations of the component drugs. We find that populations evolved in the hybrid gain less resistance than those evolved in an equimolar mixture of the hybrid's components, in part because the hybrid evades resistance mediated by the multiple antibiotic resistance (mar) operon. Furthermore, we find that the ciprofloxacin moiety of the hybrid inhibits bacterial growth whereas the neomycin B moiety diminishes the effectiveness of mar activation. More generally, comparing the phenotypic and genotypic paths to resistance across different drug treatments can pinpoint unique properties of new compounds that limit the emergence of resistance.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Farmacorresistência Bacteriana Múltipla
/
Antibacterianos
Idioma:
En
Revista:
Mol Biol Evol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Marrocos