A spectral approach for the quantitative description of cardiac collagen network from nonlinear optical imaging.
Annu Int Conf IEEE Eng Med Biol Soc
; 2015: 6257-60, 2015.
Article
em En
| MEDLINE
| ID: mdl-26737722
ABSTRACT
The assessment of collagen structure in cardiac pathology, such as atrial fibrillation (AF), is essential for a complete understanding of the disease. This paper introduces a novel methodology for the quantitative description of collagen network properties, based on the combination of nonlinear optical microscopy with a spectral approach of image processing and analysis. Second-harmonic generation (SHG) microscopy was applied to atrial tissue samples from cardiac surgery patients, providing label-free, selective visualization of the collagen structure. The spectral analysis framework, based on 2D-FFT, was applied to the SHG images, yielding a multiparametric description of collagen fiber orientation (angle and anisotropy indexes) and texture scale (dominant wavelength and peak dispersion indexes). The proof-of-concept application of the methodology showed the capability of our approach to detect and quantify differences in the structural properties of the collagen network in AF versus sinus rhythm patients. These results suggest the potential of our approach in the assessment of collagen properties in cardiac pathologies related to a fibrotic structural component.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Colágeno
/
Imagem Óptica
Limite:
Humans
Idioma:
En
Revista:
Annu Int Conf IEEE Eng Med Biol Soc
Ano de publicação:
2015
Tipo de documento:
Article