Long non-coding RNA expression in primary human monocytes.
Genomics
; 108(1): 37-45, 2016 07.
Article
em En
| MEDLINE
| ID: mdl-26778813
ABSTRACT
Long non-coding RNAs (lncRNAs) have been shown to possess a wide range of functions in both cellular and developmental processes including cancers. Although some of the lncRNAs have been implicated in the regulation of the immune response, the exact function of the large majority of lncRNAs still remains unknown. In this study, we characterized the lncRNAs in human primary monocytes, an essential component of the innate immune system. We performed RNA sequencing of monocytes from four individuals and combined our data with eleven other publicly available datasets. Our analysis led to identification of ~8000 lncRNAs of which >1000 have not been previously reported in monocytes. PCR-based validation of a subset of the identified novel long intergenic noncoding RNAs (lincRNAs) revealed distinct expression patterns. Our study provides a landscape of lncRNAs in monocytes, which could facilitate future experimental studies to characterize the functions of these molecules in the innate immune system.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Monócitos
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Análise de Sequência de RNA
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Perfilação da Expressão Gênica
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Transcriptoma
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RNA Longo não Codificante
Limite:
Humans
Idioma:
En
Revista:
Genomics
Assunto da revista:
GENETICA
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Malásia