Validation of downregulated microRNAs during osteoclast formation and osteoporosis progression.
Mol Med Rep
; 13(3): 2273-80, 2016 Mar.
Article
em En
| MEDLINE
| ID: mdl-26783047
ABSTRACT
Enhanced osteoclast formation and function have essential roles during postmenopausal osteoporosis. A number of cytokines have been reported to regulate osteoclastogenesis and to be involved during the pathogenesis of osteoporosis. However, the regulation of osteolysis by microRNAs (miRNAs) has remained to be fully elucidated. The present study used a microarray analysis to identify a variety of miRNAs that are differentially expressed during osteoclast formation. Six downregulated miRNAs, miR21a5p, miR27a3p, let7i5p, miR223p, miR3405p and miR23a5p, whose molecular mechanisms during osteoclast differentiation have not been reported previously, were further assessed. Using an osteoclast formation assay and a mouse model of progressive osteoporosis, the downregulation of these miRNAs was validated in vitro and in vivo. Of note, the expression patterns of these six miRNAs were associated with the progression of osteoporosis. Therefore, these miRNAs are of potential diagnostic and therapeutic value for osteolytic diseases.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Osteoclastos
/
Osteoporose
/
Regulação para Baixo
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Progressão da Doença
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MicroRNAs
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Mol Med Rep
Ano de publicação:
2016
Tipo de documento:
Article