Suppression of cFLIPL promotes JNK activation in malignant melanoma cells.
Mol Med Rep
; 13(3): 2904-8, 2016 Mar.
Article
em En
| MEDLINE
| ID: mdl-26847085
ABSTRACT
The upregulation of cellular Fasassociated death domainlike interleukin1ßconverting enzyme (FLICE)like inhibitory protein (cFLIP) has been reported in various tumor types, and has been previously shown to be associated with the clinicopathological features of melanoma. To assess its potential role in cancer therapy, the present study evaluated the effects of short hairpin (sh)RNAs of different cFLIP isoforms on cellular proliferation and cJun Nterminal kinase (JNK) signaling. Human cFLIP shRNA plasmids were constructed and transfected into the A875 melanoma cell line. It was observed that cFLIP shRNA exhibited strong inhibitory effects against the levels of phosphorylatedJNK and inhibited cellular proliferation in A875 cells. Thus, this indicated that cFLIP long form shRNA serves a specific inhibitory role in cellular proliferation through inducing the activation of the JNK pathway in A875 cells. The present study provided insight into the development of RNAi based therapies for melanoma.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Proteína Reguladora de Apoptosis Semelhante a CASP8 e FADD
/
Melanoma
Limite:
Humans
Idioma:
En
Revista:
Mol Med Rep
Ano de publicação:
2016
Tipo de documento:
Article