Pathogen receptor discovery with a microfluidic human membrane protein array.
Proc Natl Acad Sci U S A
; 113(16): 4344-9, 2016 Apr 19.
Article
em En
| MEDLINE
| ID: mdl-27044079
ABSTRACT
The discovery of how a pathogen invades a cell requires one to determine which host cell receptors are exploited. This determination is a challenging problem because the receptor is invariably a membrane protein, which represents an Achilles heel in proteomics. We have developed a universal platform for high-throughput expression and interaction studies of membrane proteins by creating a microfluidic-based comprehensive human membrane protein array (MPA). The MPA is, to our knowledge, the first of its kind and offers a powerful alternative to conventional proteomics by enabling the simultaneous study of 2,100 membrane proteins. We characterized direct interactions of a whole nonenveloped virus (simian virus 40), as well as those of the hepatitis delta enveloped virus large form antigen, with candidate host receptors expressed on the MPA. Selected newly discovered membrane protein-pathogen interactions were validated by conventional methods, demonstrating that the MPA is an important tool for cellular receptor discovery and for understanding pathogen tropism.
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Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Receptores Virais
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Vírus 40 dos Símios
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Análise Serial de Proteínas
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Proteômica
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Técnicas Analíticas Microfluídicas
Limite:
Humans
Idioma:
En
Revista:
Proc Natl Acad Sci U S A
Ano de publicação:
2016
Tipo de documento:
Article