Ultraviolet B irradiation in the prevention of alloimmunization in a dog platelet transfusion model.
Transfusion
; 56(6): 1340-7, 2016 06.
Article
em En
| MEDLINE
| ID: mdl-27184061
ABSTRACT
BACKGROUND:
Alloimmune platelet (PLT) refractoriness remains a significant problem for chronically transfused patients with thrombocytopenia. STUDY DESIGN ANDMETHODS:
In a dog PLT transfusion model, we evaluated ultraviolet B irradiation (UV-B) of donor PLTs-either alone or in combination with centrifuge leukoreduction (C-LR) or filtration leukoreduction (F-LR)-to prevent refractoriness to donor PLTs and to induce tolerance to standard (STD) PLTs from the same donor or to tertiary donors.RESULTS:
Recipient acceptance rates for C-LR donor PLT transfusions were 14%, F-LR were 33%, and UV-B irradiated were 45% with no significant differences among the treatments given to the donor's PLTs. Adding UV-B irradiation to C-LR or F-LR PLTs increased acceptance rates to 50 and 68% (p = 0.02 and p = 0.05), respectively, comparing single treatments to the combined treatments. After a recipient had accepted any type of UV-B-treated donor PLTs, specific tolerance to subsequent transfusions of the same donor's STD PLTs averaged 65%. Nonspecific tolerance to third-party donor's STD PLTs averaged 36% if they had accepted their initial donor's treated PLTs but was only 4% (p < 0.001) if they had rejected these PLTs.CONCLUSION:
Combining UV-B irradiation with a method of leukoreduction produces additive effects on prevention of alloimmune PLT refractoriness.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Raios Ultravioleta
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Transfusão de Plaquetas
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Tolerância Imunológica
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Isoanticorpos
Limite:
Animals
Idioma:
En
Revista:
Transfusion
Ano de publicação:
2016
Tipo de documento:
Article