Mass spectrometry-based absolute quantification reveals rhythmic variation of mouse circadian clock proteins.

Narumi, Ryohei; Shimizu, Yoshihiro; Ukai-Tadenuma, Maki; Ode, Koji L; Kanda, Genki N; Shinohara, Yuta; Sato, Aya; Matsumoto, Katsuhiko; Ueda, Hiroki R

Narumi, Ryohei; Shimizu, Yoshihiro; Ukai-Tadenuma, Maki; Ode, Koji L; Kanda, Genki N; Shinohara, Yuta; Sato, Aya; Matsumoto, Katsuhiko; Ueda, Hiroki R.

Afiliação

Narumi R; Laboratory for Synthetic Biology, RIKEN Quantitative Biology Center, Suita, Osaka 565-0874, Japan;
Shimizu Y; Laboratory for Cell-Free Protein Synthesis, RIKEN Quantitative Biology Center, Suita, Osaka 565-0874, Japan; yshimizu@riken.jp uedah-tky@umin.ac.jp.
Ukai-Tadenuma M; Laboratory for Synthetic Biology, RIKEN Quantitative Biology Center, Suita, Osaka 565-0874, Japan;
Ode KL; Department of Systems Pharmacology, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan;
Kanda GN; Laboratory for Synthetic Biology, RIKEN Quantitative Biology Center, Suita, Osaka 565-0874, Japan; Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565-0871, Japan;
Shinohara Y; Laboratory for Synthetic Biology, RIKEN Quantitative Biology Center, Suita, Osaka 565-0874, Japan; Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565-0871, Japan;
Sato A; Laboratory for Cell-Free Protein Synthesis, RIKEN Quantitative Biology Center, Suita, Osaka 565-0874, Japan;
Matsumoto K; Laboratory for Synthetic Biology, RIKEN Quantitative Biology Center, Suita, Osaka 565-0874, Japan;
Ueda HR; Laboratory for Synthetic Biology, RIKEN Quantitative Biology Center, Suita, Osaka 565-0874, Japan; Department of Systems Pharmacology, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan; Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565-0

Proc Natl Acad Sci U S A ; 113(24): E3461-7, 2016 06 14.

Article em En | MEDLINE | ID: mdl-27247408

Assuntos

Peptídeos e Proteínas de Sinalização do Ritmo Circadiano; Ritmo Circadiano/fisiologia; Espectrometria de Massas/métodos; Animais; Peptídeos e Proteínas de Sinalização do Ritmo Circadiano/análise; Peptídeos e Proteínas de Sinalização do Ritmo Circadiano/genética; Peptídeos e Proteínas de Sinalização do Ritmo Circadiano/metabolismo; Masculino; Camundongos; Camundongos Knockout

Palavras-chave

absolute quantification; cell-free protein synthesis system; mammalian circadian clock protein; mass spectrometry; targeted proteomics

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Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Espectrometria de Massas / Ritmo Circadiano / Peptídeos e Proteínas de Sinalização do Ritmo Circadiano Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Proc Natl Acad Sci U S A Ano de publicação: 2016 Tipo de documento: Article

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