Combining reverse-transcription multiplex PCR and microfluidic electrophoresis to simultaneously detect seven mosquito-transmitted zoonotic encephalomyelitis viruses.
Vet J
; 212: 27-35, 2016 Jun.
Article
em En
| MEDLINE
| ID: mdl-27256022
ABSTRACT
Several mosquito-transmitted viruses are causative agents for zoonotic encephalomyelitis. Rapid identification of these viruses in mosquito populations is an effective method for surveying these diseases. To detect multiple mosquito-transmitted viral agents, including West Nile virus, Saint Louis encephalitis virus, Venezuelan equine encephalomyelitis virus, Western equine encephalomyelitis virus, Eastern equine encephalomyelitis virus, Highlands J virus and Japanese encephalitis virus, an assay using multiplex reverse-transcription PCR combined with microfluidic electrophoresis was developed and evaluated. Tailed nested primers were used in the assay to amplify specific viral genomic segments, and products with specific length were further analyzed by using a microfluidic electrophoresis chip. The assay exhibited good specificity and analytical sensitivity (10(2) copies/µL). This technology can be helpful in the quarantine and surveillance of exotic encephalomyelitis viruses which are transmitted by mosquitoes.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Reação em Cadeia da Polimerase Via Transcriptase Reversa
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Eletroforese em Microchip
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Vírus da Encefalite
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Reação em Cadeia da Polimerase Multiplex
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Monitoramento Epidemiológico
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Culicidae
Tipo de estudo:
Screening_studies
Limite:
Animals
Idioma:
En
Revista:
Vet J
Assunto da revista:
MEDICINA VETERINARIA
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
China