Over 20% (15)N Hyperpolarization in Under One Minute for Metronidazole, an Antibiotic and Hypoxia Probe.
J Am Chem Soc
; 138(26): 8080-3, 2016 07 06.
Article
em En
| MEDLINE
| ID: mdl-27321159
ABSTRACT
Direct NMR hyperpolarization of naturally abundant (15)N sites in metronidazole is demonstrated using SABRE-SHEATH (Signal Amplification by Reversible Exchange in SHield Enables Alignment Transfer to Heteronuclei). In only a few tens of seconds, nuclear spin polarization P(15)N of up to â¼24% is achieved using parahydrogen with 80% para fraction corresponding to P(15)N ≈ 32% if â¼100% parahydrogen were employed (which would translate to a signal enhancement of â¼0.1-million-fold at 9.4 T). In addition to this demonstration on the directly binding (15)N site (using J(2)H-(15)N), we also hyperpolarized more distant (15)N sites in metronidazole using longer-range spin-spin couplings (J(4)H-(15)N and J(5)H-(15)N). Taken together, these results significantly expand the range of molecular structures and sites amenable to hyperpolarization via low-cost parahydrogen-based methods. In particular, hyperpolarized nitroimidazole and its derivatives have powerful potential applications such as direct in vivo imaging of mechanisms of action or hypoxia sensing.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Sondas Moleculares
/
Hipóxia Tumoral
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Metronidazol
/
Antibacterianos
Idioma:
En
Revista:
J Am Chem Soc
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Estados Unidos