Analysis of an echovirus 18 outbreak in Thuringia, Germany: insights into the molecular epidemiology and evolution of several enterovirus species B members.
Med Microbiol Immunol
; 205(5): 471-83, 2016 Oct.
Article
em En
| MEDLINE
| ID: mdl-27369854
ABSTRACT
In October and November 2010, six children and one woman were presented with symptoms of aseptic meningitis in Jena, Thuringia, Germany. Enterovirus RNA was detected in the cerebrospinal fluid of all patients by RT-PCR, and preliminary molecular typing revealed echovirus 18 (E-18) as causative agent. Virus isolates were obtained from stool samples of three patients and several contact persons. Again, most isolates were typed as E-18. In addition, coxsackievirus B5 (CV-B5) and echovirus 25 (E-25) were found to co-circulate. As only few complete E-18 sequences are available in GenBank, the entire genomes of these isolates were determined using direct RNA-sequencing technology. We did not find evidence for recombination between E-18, E-25 or CV-B5 during the outbreak. Viral protein 1 gene sequences and the cognate 3D polymerase gene sequences of each isolate and GenBank sequences were analysed in order to define type-specific recombination groups (recogroups).
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Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Surtos de Doenças
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Enterovirus Humano B
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Infecções por Echovirus
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Meningite Asséptica
Tipo de estudo:
Screening_studies
Limite:
Adult
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Child
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Child, preschool
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Female
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Humans
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Male
País/Região como assunto:
Europa
Idioma:
En
Revista:
Med Microbiol Immunol
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Alemanha