Pharmacokinetic parameters of three active ingredients hederacoside C, hederacoside D, and É-hederin in Hedera helix in rats.
J Sep Sci
; 39(17): 3292-301, 2016 Sep.
Article
em En
| MEDLINE
| ID: mdl-27377040
ABSTRACT
In Hedera helix hederacoside C, hederacoside D, and É-hederin are three major bioactive saponins and play pivotal roles in the overall biological activity. In this study, a specific and sensitive ultra-high performance liquid chromatography with tandem mass spectrometry method has been developed and validated for the quantification of three major bioactive saponins in rat plasma. Chromatographic separation was performed on a reversed-phase Thermo Hypersil GOLD C18 column (2.1 mm × 50 mm, 1.9 µm) using a gradient mobile phase system of acetonitrile-water containing 0.1% formic acid. The assay was successfully applied to study the pharmacokinetic behavior of the three analytes in rats after oral and intravenous administration of a mixture of saponins (hederacoside C, hederacoside D, and É-hederin). Further research was performed to compare the pharmacokinetic behavior of the three analytes after the oral administration of a mixture of saponins and an extract of saponins from Hedera helix, and results showed that double peaks were evident on concentration-time profile for each of the three saponins. The difference in the pharmacokinetic characteristics of three saponins between a mixture of saponins and an extract of saponins from Hedera helix was found in rat, which would be beneficial for the preclinical research and clinical use of Hedera helix.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Ácido Oleanólico
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Saponinas
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Extratos Vegetais
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Cromatografia Líquida de Alta Pressão
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Hedera
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Espectrometria de Massas em Tandem
Tipo de estudo:
Evaluation_studies
Limite:
Animals
Idioma:
En
Revista:
J Sep Sci
Ano de publicação:
2016
Tipo de documento:
Article