Virtual microfluidics for digital quantification and single-cell sequencing.
Nat Methods
; 13(9): 759-62, 2016 09.
Article
em En
| MEDLINE
| ID: mdl-27479330
ABSTRACT
We have developed hydrogel-based virtual microfluidics as a simple and robust alternative to complex engineered microfluidic systems for the compartmentalization of nucleic acid amplification reactions. We applied in-gel digital multiple displacement amplification (dMDA) to purified DNA templates, cultured bacterial cells and human microbiome samples in the virtual microfluidics system, and demonstrated whole-genome sequencing of single-cell MDA products with excellent coverage uniformity and markedly reduced chimerism compared with products of liquid MDA reactions.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Genoma Bacteriano
/
Análise de Sequência de DNA
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Técnicas de Amplificação de Ácido Nucleico
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Microfluídica
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Análise de Célula Única
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Sequenciamento de Nucleotídeos em Larga Escala
Idioma:
En
Revista:
Nat Methods
Assunto da revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Estados Unidos