Delivery of RNA-based molecules to human hematopoietic stem and progenitor cells for modulation of gene expression.
Exp Hematol
; 44(11): 991-1001, 2016 Nov.
Article
em En
| MEDLINE
| ID: mdl-27576131
ABSTRACT
Gene modulation of human hematopoietic stem and progenitor cells (HSPCs) harbors great potential for therapeutic application of these cells and presents a versatile tool in basic research to enhance our understanding of HSPC biology. However, stable genetic modification might be adverse, particularly in clinical settings. Here, we review a broad range of approaches to transient, nonviral modulation of protein expression with a focus on RNA-based methods. We compare different delivery methods and describe the usefulness of RNA molecules for overexpression as well as downregulation of proteins in HSPCs.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
RNA
/
Células-Tronco Hematopoéticas
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Expressão Gênica
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Técnicas de Transferência de Genes
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Engenharia Celular
Limite:
Humans
Idioma:
En
Revista:
Exp Hematol
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Alemanha