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A global genetic interaction network maps a wiring diagram of cellular function.
Costanzo, Michael; VanderSluis, Benjamin; Koch, Elizabeth N; Baryshnikova, Anastasia; Pons, Carles; Tan, Guihong; Wang, Wen; Usaj, Matej; Hanchard, Julia; Lee, Susan D; Pelechano, Vicent; Styles, Erin B; Billmann, Maximilian; van Leeuwen, Jolanda; van Dyk, Nydia; Lin, Zhen-Yuan; Kuzmin, Elena; Nelson, Justin; Piotrowski, Jeff S; Srikumar, Tharan; Bahr, Sondra; Chen, Yiqun; Deshpande, Raamesh; Kurat, Christoph F; Li, Sheena C; Li, Zhijian; Usaj, Mojca Mattiazzi; Okada, Hiroki; Pascoe, Natasha; San Luis, Bryan-Joseph; Sharifpoor, Sara; Shuteriqi, Emira; Simpkins, Scott W; Snider, Jamie; Suresh, Harsha Garadi; Tan, Yizhao; Zhu, Hongwei; Malod-Dognin, Noel; Janjic, Vuk; Przulj, Natasa; Troyanskaya, Olga G; Stagljar, Igor; Xia, Tian; Ohya, Yoshikazu; Gingras, Anne-Claude; Raught, Brian; Boutros, Michael; Steinmetz, Lars M; Moore, Claire L; Rosebrock, Adam P.
Afiliação
  • Costanzo M; The Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
  • VanderSluis B; Department of Computer Science and Engineering, University of Minnesota-Twin Cities, 200 Union Street, Minneapolis, MN 55455, USA. Simons Center for Data Analysis, Simons Foundation, 160 Fifth Avenue, New York, NY 10010, USA.
  • Koch EN; Department of Computer Science and Engineering, University of Minnesota-Twin Cities, 200 Union Street, Minneapolis, MN 55455, USA.
  • Baryshnikova A; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544, USA.
  • Pons C; Department of Computer Science and Engineering, University of Minnesota-Twin Cities, 200 Union Street, Minneapolis, MN 55455, USA.
  • Tan G; The Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
  • Wang W; Department of Computer Science and Engineering, University of Minnesota-Twin Cities, 200 Union Street, Minneapolis, MN 55455, USA.
  • Usaj M; The Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
  • Hanchard J; The Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1. Department of Molecular Genetics, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
  • Lee SD; Department of Developmental, Molecular and Chemical Biology, Tufts University School of Medicine, Boston, MA 02111, USA.
  • Pelechano V; European Molecular Biology Laboratory (EMBL), Genome Biology Unit, 69117 Heidelberg, Germany.
  • Styles EB; The Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1. Department of Molecular Genetics, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
  • Billmann M; Division of Signaling and Functional Genomics, German Cancer Research Center (DKFZ) and Heidelberg University, Heidelberg, Germany.
  • van Leeuwen J; The Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
  • van Dyk N; The Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
  • Lin ZY; Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto ON, Canada.
  • Kuzmin E; The Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1. Department of Molecular Genetics, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
  • Nelson J; Department of Computer Science and Engineering, University of Minnesota-Twin Cities, 200 Union Street, Minneapolis, MN 55455, USA. Program in Biomedical Informatics and Computational Biology, University of Minnesota-Twin Cities, 200 Union Street, Minneapolis, MN 55455, USA.
  • Piotrowski JS; The Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1. Chemical Genomics Research Group, RIKEN Center for Sustainable Resource Sciences (CSRS), Saitama, Japan.
  • Srikumar T; Princess Margaret Cancer Centre, University Health Network and Department of Medical Biophysics, University of Toronto, Toronto ON, Canada.
  • Bahr S; The Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
  • Chen Y; The Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
  • Deshpande R; Department of Computer Science and Engineering, University of Minnesota-Twin Cities, 200 Union Street, Minneapolis, MN 55455, USA.
  • Kurat CF; The Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
  • Li SC; The Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1. Chemical Genomics Research Group, RIKEN Center for Sustainable Resource Sciences (CSRS), Saitama, Japan.
  • Li Z; The Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
  • Usaj MM; The Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
  • Okada H; Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Chiba, Japan 277-8561.
  • Pascoe N; The Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1. Department of Molecular Genetics, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
  • San Luis BJ; The Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
  • Sharifpoor S; The Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
  • Shuteriqi E; The Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
  • Simpkins SW; Department of Computer Science and Engineering, University of Minnesota-Twin Cities, 200 Union Street, Minneapolis, MN 55455, USA. Program in Biomedical Informatics and Computational Biology, University of Minnesota-Twin Cities, 200 Union Street, Minneapolis, MN 55455, USA.
  • Snider J; The Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
  • Suresh HG; The Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
  • Tan Y; The Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
  • Zhu H; The Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
  • Malod-Dognin N; Computer Science Deptartment, University College London, London WC1E 6BT, UK.
  • Janjic V; Department of Computing, Imperial College London, UK.
  • Przulj N; Computer Science Deptartment, University College London, London WC1E 6BT, UK. School of Computing (RAF), Union University, Belgrade, Serbia.
  • Troyanskaya OG; Simons Center for Data Analysis, Simons Foundation, 160 Fifth Avenue, New York, NY 10010, USA. Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544, USA.
  • Stagljar I; The Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1. Department of Molecular Genetics, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1. Department of Biochemistry, University of Toronto, Toronto, ON, Canada.
  • Xia T; Department of Computer Science and Engineering, University of Minnesota-Twin Cities, 200 Union Street, Minneapolis, MN 55455, USA. School of Electronic Information and Communications, Huazhong University of Science and Technology, Wuhan, China, 430074.
  • Ohya Y; Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Chiba, Japan 277-8561.
  • Gingras AC; Department of Molecular Genetics, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1. Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto ON, Canada.
  • Raught B; Princess Margaret Cancer Centre, University Health Network and Department of Medical Biophysics, University of Toronto, Toronto ON, Canada.
  • Boutros M; Division of Signaling and Functional Genomics, German Cancer Research Center (DKFZ) and Heidelberg University, Heidelberg, Germany.
  • Steinmetz LM; European Molecular Biology Laboratory (EMBL), Genome Biology Unit, 69117 Heidelberg, Germany. Department of Genetics, School of Medicine and Stanford Genome Technology Center Stanford University, Palo Alto, CA 94304, USA.
  • Moore CL; Department of Developmental, Molecular and Chemical Biology, Tufts University School of Medicine, Boston, MA 02111, USA.
  • Rosebrock AP; The Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1. Department of Molecular Genetics, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
Science ; 353(6306)2016 09 23.
Article em En | MEDLINE | ID: mdl-27708008
ABSTRACT
We generated a global genetic interaction network for Saccharomyces cerevisiae, constructing more than 23 million double mutants, identifying about 550,000 negative and about 350,000 positive genetic interactions. This comprehensive network maps genetic interactions for essential gene pairs, highlighting essential genes as densely connected hubs. Genetic interaction profiles enabled assembly of a hierarchical model of cell function, including modules corresponding to protein complexes and pathways, biological processes, and cellular compartments. Negative interactions connected functionally related genes, mapped core bioprocesses, and identified pleiotropic genes, whereas positive interactions often mapped general regulatory connections among gene pairs, rather than shared functionality. The global network illustrates how coherent sets of genetic interactions connect protein complex and pathway modules to map a functional wiring diagram of the cell.
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Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Saccharomyces cerevisiae / Proteínas de Saccharomyces cerevisiae / Redes Reguladoras de Genes / Pleiotropia Genética / Genes Fúngicos Idioma: En Revista: Science Ano de publicação: 2016 Tipo de documento: Article
Texto completo
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XML
PubMed Links
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Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Saccharomyces cerevisiae / Proteínas de Saccharomyces cerevisiae / Redes Reguladoras de Genes / Pleiotropia Genética / Genes Fúngicos Idioma: En Revista: Science Ano de publicação: 2016 Tipo de documento: Article