VEGF regulates relative allocation of Isl1+ cardiac progenitors to myocardial and endocardial lineages.
Mech Dev
; 142: 40-49, 2016 11.
Article
em En
| MEDLINE
| ID: mdl-27794491
ABSTRACT
A fundamental issue in organogenesis is how dichotomous fate decisions are made securing proper allocation of multipotent progenitors to their respective descendants. Previous lineage tracing analyses showing Isl1+/VEGFR2+ cardiac progenitors in the second heart field give rise to both endocardium and myocardium suggest VEGF plays a role in this fate decision, conceivably promoting an endocardial fate. Isl1+ multipotent progenitors and lineage-committed descendants thereof were visualized and quantified within their transition zone in the outflow tract. Forced VEGF expression during the critical E8.5-E10.5 interval tilted the balance between myocardial- and endocardial-committed progenitors towards the latter, culminating in generation of surplus endocardium developing at the expense of a much thinner myocardium. Experiments ruled-out that surplus endocardium is due to VEGF-induced endocardial proliferation and that reduced myocardium is due to myocardial apoptosis. Inducing VEGF after most Isl1+ progenitors have been exhausted had no effect on the normal endocardia/myocardial ratio but instead produced an unrelated coronary phenotype. Together, these results uncover a novel role for VEGF in controlling proper allocation of Isl1+ cardiac progenitors to their respective descending lineages.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Fatores de Transcrição
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Organogênese
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Fator A de Crescimento do Endotélio Vascular
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Proliferação de Células
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Proteínas com Homeodomínio LIM
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Mech Dev
Assunto da revista:
EMBRIOLOGIA
Ano de publicação:
2016
Tipo de documento:
Article