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Brominated Luciferins Are Versatile Bioluminescent Probes.
Steinhardt, Rachel C; Rathbun, Colin M; Krull, Brandon T; Yu, Jason M; Yang, Yuhang; Nguyen, Brian D; Kwon, Jake; McCutcheon, David C; Jones, Krysten A; Furche, Filipp; Prescher, Jennifer A.
Afiliação
  • Steinhardt RC; Department of Chemistry, University of California, Irvine, 1120 Natural Sciences II, Irvine, CA, 92697, USA.
  • Rathbun CM; Department of Chemistry, University of California, Irvine, 1120 Natural Sciences II, Irvine, CA, 92697, USA.
  • Krull BT; Department of Chemistry, University of California, Irvine, 1120 Natural Sciences II, Irvine, CA, 92697, USA.
  • Yu JM; Department of Chemistry, University of California, Irvine, 1120 Natural Sciences II, Irvine, CA, 92697, USA.
  • Yang Y; Department of Chemistry, University of California, Irvine, 1120 Natural Sciences II, Irvine, CA, 92697, USA.
  • Nguyen BD; Department of Chemistry, University of California, Irvine, 1120 Natural Sciences II, Irvine, CA, 92697, USA.
  • Kwon J; Department of Chemistry, University of California, Irvine, 1120 Natural Sciences II, Irvine, CA, 92697, USA.
  • McCutcheon DC; Department of Chemistry, University of California, Irvine, 1120 Natural Sciences II, Irvine, CA, 92697, USA.
  • Jones KA; Department of Molecular Biology and Biochemistry, University of California, Irvine, 3205 McGaugh Hall, Irvine, CA, 92697, USA.
  • Furche F; Department of Chemistry, University of California, Irvine, 1120 Natural Sciences II, Irvine, CA, 92697, USA.
  • Prescher JA; Department of Chemistry, University of California, Irvine, 1120 Natural Sciences II, Irvine, CA, 92697, USA.
Chembiochem ; 18(1): 96-100, 2017 Jan 03.
Article em En | MEDLINE | ID: mdl-27930848
ABSTRACT
We report a set of brominated luciferins for bioluminescence imaging. These regioisomeric scaffolds were accessed by using a common synthetic route. All analogues produced light with firefly luciferase, although varying levels of emission were observed. Differences in photon output were analyzed by computation and photophysical measurements. The brightest brominated luciferin was further evaluated in cell and animal models. At low doses, the analogue outperformed the native substrate in cells. The remaining luciferins, although weak emitters with firefly luciferase, were inherently capable of light production and thus potential substrates for orthogonal mutant enzymes.
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Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Luciferina de Vaga-Lumes / Medições Luminescentes Limite: Animals / Humans Idioma: En Revista: Chembiochem Assunto da revista: BIOQUIMICA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Luciferina de Vaga-Lumes / Medições Luminescentes Limite: Animals / Humans Idioma: En Revista: Chembiochem Assunto da revista: BIOQUIMICA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Estados Unidos