miR-194 functions as a novel modulator of cellular senescence in mouse embryonic fibroblasts.
Cell Biol Int
; 41(3): 249-257, 2017 Mar.
Article
em En
| MEDLINE
| ID: mdl-27981676
ABSTRACT
MicroRNA-194 (miR-194), a typical p53 responsive miRNA, serves as a tumor suppressor similar as p53, and has been demonstrated to play an anti-proliferation role in various human cancers. In spite of the pivotal role of p53 during aging process, the knowledge of miR-194's contribution to cellular senescence is limited. We herein sought to explore the role of miR-194 in the replicative senescence and stress-induced senescence of mouse embryonic fibroblasts. Our results unraveled that, compared to young cells, miR-194 is highly expressed in senescent cells, and extra expression of miR-194 significantly triggers the replicative senescence of MEFs and H2 O2 -induced senescence of NIH/3T3 cells, while inhibition of miR-194 exhibited the opposite effect. We further unveiled that DNMT3A was a direct and authentic target of miR-194, which has been reported to be closely associated with cellular senescence. Taken together, our data suggest that miR-194 may significantly promote the development of cellular senescence in mouse embryonic fibroblasts, which potentially occurs through inhibiting the DNMT3A expression.
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Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Senescência Celular
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MicroRNAs
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Fibroblastos
Limite:
Animals
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Humans
Idioma:
En
Revista:
Cell Biol Int
Ano de publicação:
2017
Tipo de documento:
Article