Phytochemical Analysis of a Cytotoxic Fraction of Quassia silvestris using LC-HR-ESI-MSn.
Phytochem Anal
; 28(3): 210-216, 2017 May.
Article
em En
| MEDLINE
| ID: mdl-28028887
ABSTRACT
INTRODUCTION:
The genus Quassia is a promising source of secondary metabolites with biological potential including antimalarial and cytotoxic activities. Limited data are available on the phytochemistry and pharmacology of Quassia silvestris Cheek & Jongkind, a Cameroonian medicinal plant used to treat various ailments.OBJECTIVES:
To carry out the bioassay-guided fractionation and LC-HR-ESI-MS analyses of the leaves extract from Q. silvestris; to purify the active fractions and isolate the major compounds using different chromatographic and spectroscopic methods. The obtained compounds will be evaluated for their biological activity. MATERIAL ANDMETHODS:
Following the cytotoxic screening and LC-HR-ESI-MS profiling of fractions obtained from partition of the methanolic extract of Q. silvestris leaves, the CH2 Cl2 -soluble fraction which exhibited the highest cytotoxicity was retained for further investigations.RESULTS:
Sixteen squalene-derived metabolites were identified with oxasqualenoid derivatives being the most predominant. Among the isolates, structure elucidation of two new oxasqualenoids quassiols E (1) and F (2), were achieved by NMR (one-dimensional (1D) and two-dimensional (2D)) and MS methods. The newly characterised compounds 1 and 2, together with the known tetraol (3) and 3-oxo-oleanoic acid (16) displayed moderate cytotoxicity.CONCLUSION:
The identification and structural characterisation of highly oxidised squalene derived metabolites from this plant may provide important insight data for further pharmacological investigations. The LC-HR-ESI-MSn method reported here could be developed as a rapid and efficient tool for the analyses of structurally related compounds in the genera Quassia, Simarouba, and Eurycoma of the subfamily Simarouboideae. Copyright © 2016 John Wiley & Sons, Ltd.Palavras-chave
Texto completo:
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Bases de dados:
MEDLINE
Assunto principal:
Cromatografia Líquida
/
Espectrometria de Massas por Ionização por Electrospray
/
Quassia
/
Antineoplásicos Fitogênicos
Limite:
Humans
Idioma:
En
Revista:
Phytochem Anal
Assunto da revista:
BOTANICA
/
QUIMICA
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Camarões