Paenibacillus rhizoplanae sp. nov., isolated from the rhizosphere of Zea mays.
Int J Syst Evol Microbiol
; 67(4): 1058-1063, 2017 Apr.
Article
em En
| MEDLINE
| ID: mdl-28068214
ABSTRACT
A Gram-stain-positive, aerobic, endospore-forming bacterial strain isolated from the rhizosphere of Zea mays was studied to determine its detailed taxonomic position. Based on 16S rRNA gene sequence similarity comparisons, strain JJ-64T was shown to be a member of the genus Paenibacillus, most closely related to the type strains of Paenibacillus silagei (99â%) and Paenibacillus borealis (97.5â%). 16S rRNA gene sequence similarity to all other Paenibacillus species was ≤97.5â%. DNA-DNA hybridization values to the type strains of P. silagei and P. borealis were 51â% (reciprocal 25â%) and 31â% (reciprocal 37â%), respectively. The presence of meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan, the major quinone MK-7 and the polyamine pattern with spermidine as the major component were well in line with the characteristics of the genus Paenibacillus. Furthermore, the polar lipid profile of strain JJ-64T with the predominant lipids diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylserine and two unidentified aminophospholipids reflected the close phylogenetic relatedness to P. silagei. Major fatty acids were iso- and anteiso-branched components. Physiological and biochemical characteristics allowed the further phenotypic differentiation of strain JJ-64T from the most closely related species. Thus, strain JJ-64T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus rhizoplanae sp. nov. is proposed. The type strain is JJ-64T (=LMG 29875T=CCM 8725T).
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Filogenia
/
Zea mays
/
Paenibacillus
/
Rizosfera
Idioma:
En
Revista:
Int J Syst Evol Microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Alemanha