Chemically modified surface functional groups of Alcaligenes sp. S-XJ-1 to enhance its demulsifying capability.
Appl Microbiol Biotechnol
; 101(9): 3839-3848, 2017 May.
Article
em En
| MEDLINE
| ID: mdl-28091790
ABSTRACT
Cell-surface functional groups (amino, carboxyl, hydroxyl, as well as phosphate) were chemically modified in various ways to enhance the demulsification capability of the demulsifying bacteria Alcaligenes sp. S-XJ-1. Results demonstrated that the demulsifying activity was significantly inhibited by amino enrichment with cetyl trimethyl ammonium bromide, amino methylation, hydroxyl acetylation, and phosphate esterification, but was gradually promoted by carboxyl blocking with increasing the extents of esterification. Compared with the raw biomass, an optimal esterification of carboxyl moieties enhanced the demulsification ratio by 26.5% and shortened the emulsion half-life from 24 to 8.8 h. The demulsification boost was found to be dominated by strengthened hydrophobicity (from 53° to 74°) and weakened electronegativity (from -34.6 to -4.3 mV at pH 7.0) of the cell surface, allowing the rapid dispersion and adsorption of cells onto the oil-water interface. The chemical modification of the functional groups on the biomass surface is a promising tool for the creation of a high-performance bacterial demulsifier.
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Bases de dados:
MEDLINE
Assunto principal:
Propriedades de Superfície
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Alcaligenes
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Emulsões
Idioma:
En
Revista:
Appl Microbiol Biotechnol
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
China