Sequencing thousands of single-cell genomes with combinatorial indexing.
Nat Methods
; 14(3): 302-308, 2017 03.
Article
em En
| MEDLINE
| ID: mdl-28135258
ABSTRACT
Single-cell genome sequencing has proven valuable for the detection of somatic variation, particularly in the context of tumor evolution. Current technologies suffer from high library construction costs, which restrict the number of cells that can be assessed and thus impose limitations on the ability to measure heterogeneity within a tissue. Here, we present single-cell combinatorial indexed sequencing (SCI-seq) as a means of simultaneously generating thousands of low-pass single-cell libraries for detection of somatic copy-number variants. We constructed libraries for 16,698 single cells from a combination of cultured cell lines, primate frontal cortex tissue and two human adenocarcinomas, and obtained a detailed assessment of subclonal variation within a pancreatic tumor.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Neoplasias Pancreáticas
/
Adenocarcinoma
/
Mapeamento Cromossômico
/
Análise de Sequência de DNA
/
Variações do Número de Cópias de DNA
/
Análise de Célula Única
/
Sequenciamento de Nucleotídeos em Larga Escala
/
Lobo Frontal
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Nat Methods
Assunto da revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Estados Unidos