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Pathology-targeted cell delivery via injectable micro-scaffold capsule mediated by endogenous TGase.
Qi, Chunxiao; Li, Yaqian; Badger, Patrick; Yu, Hongsheng; You, Zhifeng; Yan, Xiaojun; Liu, Wei; Shi, Yan; Xia, Tie; Dong, Jiahong; Huang, Chenyu; Du, Yanan.
Afiliação
  • Qi C; Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing 100084, China.
  • Li Y; Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing 100084, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, China.
  • Badger P; Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA.
  • Yu H; Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing 100084, China.
  • You Z; Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing 100084, China.
  • Yan X; Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing 100084, China.
  • Liu W; Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing 100084, China.
  • Shi Y; Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing 100084, China.
  • Xia T; Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing 100084, China.
  • Dong J; Department of Hepatobiliary Surgery, Beijing Tsinghua Changgung Hospital, Tsinghua University, Beijing 102218, China.
  • Huang C; Department of Plastic, Reconstructive and Aesthetic Surgery, Beijing Tsinghua Changgung Hospital, Tsinghua University, Beijing 102218, China.
  • Du Y; Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing 100084, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, China. Electronic address: duyanan@tsinghua.edu.cn.
Biomaterials ; 126: 1-9, 2017 05.
Article em En | MEDLINE | ID: mdl-28237907
ABSTRACT
Targeted cell delivery to lesion sites via minimally invasive approach remains an unmet need in regenerative medicine to endow satisfactory therapeutic efficacy and minimized side-effects. Here, we rationally designed a pathology-targeted cell delivery strategy leveraging injectable micro-scaffolds as cell-loading capsule and endogenous tissue transglutaminase (TGase) at lesion site as adhesive. Up-regulated TGase post-liver injury catalyzed chemical bonding between the glutamine and lysine residues on liver surface and micro-scaffolds both ex vivo and in vivo, facilitating sufficient adhesion on the pathological liver. Upon intraperitoneal injection, Mesenchymal Stem Cell-loaded capsules, exhibiting cell protection from shear-induced damage and post-transplantation anoikis, adhered to the CCl4-treated liver with a hundred-fold improvement in targeting efficiency (70.72%) compared to free-cell injection, which dramatically improved mice survival (33.3% vs. 0% for free-cell therapy) even with low-dosage treatment. This unique and widely-applicable cell delivery mechanism and strategy hold great promise for transforming cell therapy for refractory diseases.
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Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Transglutaminases / Sistemas de Liberação de Medicamentos / Proteínas de Ligação ao GTP / Alicerces Teciduais / Injeções Limite: Animals / Humans Idioma: En Revista: Biomaterials Ano de publicação: 2017 Tipo de documento: Article País de afiliação: China
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Transglutaminases / Sistemas de Liberação de Medicamentos / Proteínas de Ligação ao GTP / Alicerces Teciduais / Injeções Limite: Animals / Humans Idioma: En Revista: Biomaterials Ano de publicação: 2017 Tipo de documento: Article País de afiliação: China