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Drop-on-demand sample delivery for studying biocatalysts in action at X-ray free-electron lasers.
Fuller, Franklin D; Gul, Sheraz; Chatterjee, Ruchira; Burgie, E Sethe; Young, Iris D; Lebrette, Hugo; Srinivas, Vivek; Brewster, Aaron S; Michels-Clark, Tara; Clinger, Jonathan A; Andi, Babak; Ibrahim, Mohamed; Pastor, Ernest; de Lichtenberg, Casper; Hussein, Rana; Pollock, Christopher J; Zhang, Miao; Stan, Claudiu A; Kroll, Thomas; Fransson, Thomas; Weninger, Clemens; Kubin, Markus; Aller, Pierre; Lassalle, Louise; Bräuer, Philipp; Miller, Mitchell D; Amin, Muhamed; Koroidov, Sergey; Roessler, Christian G; Allaire, Marc; Sierra, Raymond G; Docker, Peter T; Glownia, James M; Nelson, Silke; Koglin, Jason E; Zhu, Diling; Chollet, Matthieu; Song, Sanghoon; Lemke, Henrik; Liang, Mengning; Sokaras, Dimosthenis; Alonso-Mori, Roberto; Zouni, Athina; Messinger, Johannes; Bergmann, Uwe; Boal, Amie K; Bollinger, J Martin; Krebs, Carsten; Högbom, Martin; Phillips, George N.
Afiliação
  • Fuller FD; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
  • Gul S; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
  • Chatterjee R; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
  • Burgie ES; Department of Biology, Washington University in St. Louis, St. Louis, Missouri, USA.
  • Young ID; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
  • Lebrette H; Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden.
  • Srinivas V; Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden.
  • Brewster AS; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
  • Michels-Clark T; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
  • Clinger JA; Department of BioSciences, Rice University, Houston, Texas, USA.
  • Andi B; National Synchrotron Light Source II, Brookhaven National Laboratory, Upton, New York, USA.
  • Ibrahim M; Institut für Biologie, Humboldt-Universität zu Berlin, Berlin, Germany.
  • Pastor E; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
  • de Lichtenberg C; Institutionen för Kemi, Kemiskt Biologiskt Centrum, Umeå Universitet, Umeå, Sweden.
  • Hussein R; Institut für Biologie, Humboldt-Universität zu Berlin, Berlin, Germany.
  • Pollock CJ; Department of Chemistry, Pennsylvania State University, University Park, Pennsylvania, USA.
  • Zhang M; Institut für Biologie, Humboldt-Universität zu Berlin, Berlin, Germany.
  • Stan CA; Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
  • Kroll T; SSRL, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
  • Fransson T; Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
  • Weninger C; Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
  • Kubin M; LCLS, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
  • Aller P; Institute for Methods and Instrumentation on Synchrotron Radiation Research, Helmholtz Zentrum Berlin für Materialien und Energie GmbH, Berlin, Germany.
  • Lassalle L; Diamond Light Source Limited, Harwell Science and Innovation Campus, Didcot, UK.
  • Bräuer P; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
  • Miller MD; Diamond Light Source Limited, Harwell Science and Innovation Campus, Didcot, UK.
  • Amin M; Department of Biochemistry, University of Oxford, Oxford, UK.
  • Koroidov S; Department of BioSciences, Rice University, Houston, Texas, USA.
  • Roessler CG; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
  • Allaire M; Institutionen för Kemi, Kemiskt Biologiskt Centrum, Umeå Universitet, Umeå, Sweden.
  • Sierra RG; Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
  • Docker PT; National Synchrotron Light Source II, Brookhaven National Laboratory, Upton, New York, USA.
  • Glownia JM; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
  • Nelson S; LCLS, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
  • Koglin JE; Diamond Light Source Limited, Harwell Science and Innovation Campus, Didcot, UK.
  • Zhu D; LCLS, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
  • Chollet M; LCLS, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
  • Song S; LCLS, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
  • Lemke H; LCLS, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
  • Liang M; LCLS, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
  • Sokaras D; LCLS, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
  • Alonso-Mori R; LCLS, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
  • Zouni A; LCLS, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
  • Messinger J; SSRL, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
  • Bergmann U; LCLS, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
  • Boal AK; Institut für Biologie, Humboldt-Universität zu Berlin, Berlin, Germany.
  • Bollinger JM; Institutionen för Kemi, Kemiskt Biologiskt Centrum, Umeå Universitet, Umeå, Sweden.
  • Krebs C; Department of Chemistry-Ångström, Molecular Biomimetics, Uppsala University, Uppsala, Sweden.
  • Högbom M; Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
  • Phillips GN; Department of Chemistry, Pennsylvania State University, University Park, Pennsylvania, USA.
Nat Methods ; 14(4): 443-449, 2017 Apr.
Article em En | MEDLINE | ID: mdl-28250468
ABSTRACT
X-ray crystallography at X-ray free-electron laser sources is a powerful method for studying macromolecules at biologically relevant temperatures. Moreover, when combined with complementary techniques like X-ray emission spectroscopy, both global structures and chemical properties of metalloenzymes can be obtained concurrently, providing insights into the interplay between the protein structure and dynamics and the chemistry at an active site. The implementation of such a multimodal approach can be compromised by conflicting requirements to optimize each individual method. In particular, the method used for sample delivery greatly affects the data quality. We present here a robust way of delivering controlled sample amounts on demand using acoustic droplet ejection coupled with a conveyor belt drive that is optimized for crystallography and spectroscopy measurements of photochemical and chemical reactions over a wide range of time scales. Studies with photosystem II, the phytochrome photoreceptor, and ribonucleotide reductase R2 illustrate the power and versatility of this method.
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Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Cristalografia por Raios X / Lasers Idioma: En Revista: Nat Methods Assunto da revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Cristalografia por Raios X / Lasers Idioma: En Revista: Nat Methods Assunto da revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Estados Unidos