Isolation, culture and biological characteristics of multipotent porcine skeletal muscle satellite cells.
Cell Tissue Bank
; 18(4): 513-525, 2017 Dec.
Article
em En
| MEDLINE
| ID: mdl-28255772
ABSTRACT
Skeletal muscle has a huge regenerative potential for postnatal muscle growth and repair, which mainly depends on a kind of muscle progenitor cell population, called satellite cell. Nowadays, the majority of satellite cells were obtained from human, mouse, rat and other animals but rarely from pig. In this article, the porcine skeletal muscle satellite cells were isolated and cultured in vitro. The expression of surface markers of satellite cells was detected by immunofluorescence and RT-PCR assays. The differentiation capacity was assessed by inducing satellite cells into adipocytes, myoblasts and osteoblasts. The results showed that satellite cells isolated from porcine tibialis anterior were subcultured up to 12 passages and were positive for Pax7, Myod, c-Met, desmin, PCNA and NANOG but were negative for Myogenin. Satellite cells were also induced to differentiate into adipocytes, osteoblasts and myoblasts, respectively. These findings indicated that porcine satellite cells possess similar biological characteristics of stem cells, which may provide theoretical basis and experimental evidence for potential therapeutic application in the treatment of dystrophic muscle and other muscle injuries.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Diferenciação Celular
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Separação Celular
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Músculo Esquelético
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Células Satélites de Músculo Esquelético
Limite:
Animals
Idioma:
En
Revista:
Cell Tissue Bank
Assunto da revista:
HISTOLOGIA
/
TRANSPLANTE
Ano de publicação:
2017
Tipo de documento:
Article