Your browser doesn't support javascript.
loading
M2e-tetramer-specific memory CD4 T cells are broadly protective against influenza infection.
Eliasson, D G; Omokanye, A; Schön, K; Wenzel, U A; Bernasconi, V; Bemark, M; Kolpe, A; El Bakkouri, K; Ysenbaert, T; Deng, L; Fiers, W; Saelens, X; Lycke, N.
Afiliação
  • Eliasson DG; Mucosal Immunobiology and Vaccine Center (MIVAC), Department of Microbiology and Immunology, Institute of Biomedicine, University of Gothenburg, Gothenburg, Sweden.
  • Omokanye A; Mucosal Immunobiology and Vaccine Center (MIVAC), Department of Microbiology and Immunology, Institute of Biomedicine, University of Gothenburg, Gothenburg, Sweden.
  • Schön K; Mucosal Immunobiology and Vaccine Center (MIVAC), Department of Microbiology and Immunology, Institute of Biomedicine, University of Gothenburg, Gothenburg, Sweden.
  • Wenzel UA; Mucosal Immunobiology and Vaccine Center (MIVAC), Department of Microbiology and Immunology, Institute of Biomedicine, University of Gothenburg, Gothenburg, Sweden.
  • Bernasconi V; Mucosal Immunobiology and Vaccine Center (MIVAC), Department of Microbiology and Immunology, Institute of Biomedicine, University of Gothenburg, Gothenburg, Sweden.
  • Bemark M; Mucosal Immunobiology and Vaccine Center (MIVAC), Department of Microbiology and Immunology, Institute of Biomedicine, University of Gothenburg, Gothenburg, Sweden.
  • Kolpe A; Medical Biotechnology Center, VIB and Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.
  • El Bakkouri K; Medical Biotechnology Center, VIB and Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.
  • Ysenbaert T; Medical Biotechnology Center, VIB and Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.
  • Deng L; Medical Biotechnology Center, VIB and Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.
  • Fiers W; Medical Biotechnology Center, VIB and Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.
  • Saelens X; Medical Biotechnology Center, VIB and Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.
  • Lycke N; Mucosal Immunobiology and Vaccine Center (MIVAC), Department of Microbiology and Immunology, Institute of Biomedicine, University of Gothenburg, Gothenburg, Sweden.
Mucosal Immunol ; 11(1): 273-289, 2018 01.
Article em En | MEDLINE | ID: mdl-28295019
Matrix protein 2 ectodomain (M2e) is considered an attractive component of a broadly protective, universal influenza A vaccine. Here we challenge the canonical view that antibodies against M2e are the prime effectors of protection. Intranasal immunizations of Balb/c mice with CTA1-3M2e-DD-generated M2e-specific memory CD4 T cells that were I-Ad restricted and critically protected against infection, even in the complete absence of antibodies, as observed in JhD mice. Whereas some M2e-tetramer-specific memory CD4 T cells resided in spleen and lymph nodes, the majority were lung-resident Th17 cells, that rapidly expanded upon a viral challenge infection. Indeed, immunized IL-17A-/- mice were significantly less well protected compared with wild-type mice despite exhibiting comparable antibody levels. Similarly, poor protection was also observed in congenic Balb/B (H-2b) mice, which failed to develop M2e-specific CD4 T cells, but exhibited comparable antibody levels. Lung-resident CD69+ CD103low M2e-specific memory CD4 T cells were αß TCR+ and 50% were Th17 cells that were associated with an early influx of neutrophils after virus challenge. Adoptively transferred M2e memory CD4 T cells were strong helper T cells, which accelerated M2e- but more importantly also hemagglutinin-specific IgG production. Thus, for the first time we demonstrate that M2e-specific memory CD4 T cells are broadly protective.
Assuntos

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Vírus da Influenza A / Linfócitos T CD4-Positivos / Proteínas da Matriz Viral / Infecções por Orthomyxoviridae Limite: Animals Idioma: En Revista: Mucosal Immunol Assunto da revista: ALERGIA E IMUNOLOGIA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Suécia

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Vírus da Influenza A / Linfócitos T CD4-Positivos / Proteínas da Matriz Viral / Infecções por Orthomyxoviridae Limite: Animals Idioma: En Revista: Mucosal Immunol Assunto da revista: ALERGIA E IMUNOLOGIA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Suécia