Your browser doesn't support javascript.
loading
Nuclear Membrane-Targeted Gold Nanoparticles Inhibit Cancer Cell Migration and Invasion.
Ali, Moustafa R K; Wu, Yue; Ghosh, Deepraj; Do, Brian H; Chen, Kuangcai; Dawson, Michelle R; Fang, Ning; Sulchek, Todd A; El-Sayed, Mostafa A.
Afiliação
  • Ali MRK; Laser Dynamics Lab (LDL), School of Chemistry and Biochemistry, Georgia Institute of Technology , Atlanta, Georgia 30332-0400, United States.
  • Wu Y; Laser Dynamics Lab (LDL), School of Chemistry and Biochemistry, Georgia Institute of Technology , Atlanta, Georgia 30332-0400, United States.
  • Ghosh D; Department of Molecular Pharmacology, Physiology and Biotechnology, Brown University , Providence, Rhode Island 02912, United States.
  • Do BH; School of Mechanical Engineering, Georgia Institute of Technology , Atlanta, Georgia 30332-0400, United States.
  • Chen K; Department of Chemistry, Georgia State University , P.O. Box 3965, Atlanta, Georgia 30302, United States.
  • Dawson MR; Department of Molecular Pharmacology, Physiology and Biotechnology, Brown University , Providence, Rhode Island 02912, United States.
  • Fang N; Department of Chemistry, Georgia State University , P.O. Box 3965, Atlanta, Georgia 30302, United States.
  • Sulchek TA; School of Mechanical Engineering, Georgia Institute of Technology , Atlanta, Georgia 30332-0400, United States.
  • El-Sayed MA; Laser Dynamics Lab (LDL), School of Chemistry and Biochemistry, Georgia Institute of Technology , Atlanta, Georgia 30332-0400, United States.
ACS Nano ; 11(4): 3716-3726, 2017 04 25.
Article em En | MEDLINE | ID: mdl-28333438
ABSTRACT
Most cancer patients die from metastasis. Recent studies have shown that gold nanoparticles (AuNPs) can slow down the migration/invasion speed of cancer cells and suppress metastasis. Since nuclear stiffness of the cell largely decreases cell migration, our hypothesis is that targeting AuNPs to the cell nucleus region could enhance nuclear stiffness, and therefore inhibit cell migration and invasion. Our results showed that upon nuclear targeting of AuNPs, the ovarian cancer cell motilities decrease significantly, compared with nontargeted AuNPs. Furthermore, using atomic force microscopy, we observed an enhanced cell nuclear stiffness. In order to understand the mechanism of cancer cell migration/invasion inhibition, the exact locations of the targeted AuNPs were clearly imaged using a high-resolution three-dimensional imaging microscope, which showed that the AuNPs were trapped at the nuclear membrane. In addition, we observed a greatly increased expression level of lamin A/C protein, which is located in the inner nuclear membrane and functions as a structural component of the nuclear lamina to enhance nuclear stiffness. We propose that the AuNPs that are trapped at the nuclear membrane both (1) add to the mechanical stiffness of the nucleus and (2) stimulate the overexpression of lamin A/C located around the nuclear membrane, thus increasing nuclear stiffness and slowing cancer cell migration and invasion.
Assuntos
Palavras-chave

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Neoplasias Ovarianas / Núcleo Celular / Nanopartículas Metálicas / Ouro / Antineoplásicos Limite: Female / Humans Idioma: En Revista: ACS Nano Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Neoplasias Ovarianas / Núcleo Celular / Nanopartículas Metálicas / Ouro / Antineoplásicos Limite: Female / Humans Idioma: En Revista: ACS Nano Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Estados Unidos