LMD proteomics provides evidence for hippocampus field-specific motor protein abundance changes with relevance to Alzheimer's disease.
Biochim Biophys Acta Proteins Proteom
; 1865(6): 703-714, 2017 Jun.
Article
em En
| MEDLINE
| ID: mdl-28377147
ABSTRACT
BACKGROUND:
Human hippocampal area Cornu Ammonis (CA) 1 is one of the first fields in the human telencephalon showing Alzheimer disease (AD)-specific neuropathological changes. In contrast, CA2 and CA3 are far later affected pointing to functional differences, which may be accompanied by differences in proteome endowment and changes.METHODS:
Human pyramidal cell layers of hippocampal areas CA1, CA2, and CA3 from neurologically unaffected individuals were excised using laser microdissection. The proteome of each individual sample was analyzed and differentially abundant proteins were validated by immuno-histochemistry.RESULTS:
Comparison of CA1 to CA2 revealed 223, CA1 to CA3 197 proteins with differential abundance, among them we found motor proteins MYO5A and DYNC1H1. Extension of the study to human hippocampus slices from AD patients revealed extensive depletion of these proteins in CA1 area compared to unaffected controls.CONCLUSION:
High abundance of motor proteins in pyramidal cell layers CA1 compared to CA2 and CA3 points the specific vulnerability of this hippocampal area to transport-associated changes based on microtubule dysfunction and destabilization in AD.Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Proteômica
/
Doença de Alzheimer
/
Hipocampo
/
Proteínas do Tecido Nervoso
Limite:
Aged
/
Aged80
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
Biochim Biophys Acta Proteins Proteom
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Alemanha