Quantitative microscopy-based measurements of circulating nanoparticle concentration using microliter blood volumes.
Nanomedicine
; 13(6): 1863-1867, 2017 Aug.
Article
em En
| MEDLINE
| ID: mdl-28412144
ABSTRACT
Nanoparticles (NPs) are potential drug delivery vehicles for treatment of a broad range of diseases. Intravenous (IV) administration, the most common form of delivery, is relatively non-invasive and provides (in theory) access throughout the circulatory system. However, in practice, many IV injected NPs are quickly eliminated by specialized phagocytes in the liver and spleen. Consequently, new materials have been developed with the capacity to significantly extend the circulating half-life of IV administered NPs. Unfortunately, current procedures for measuring circulation half-lives are often expensive, time consuming, and can require large blood volumes that are not compatible with mouse models of disease. Here we describe a simple and reliable procedure for measuring circulation half-life utilizing quantitative microscopy. This method requires only 2µL of blood and minimal sample preparation, yet provides robust quantitative results.
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Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Sistemas de Liberação de Medicamentos
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Nanopartículas
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Microscopia
Limite:
Animals
Idioma:
En
Revista:
Nanomedicine
Assunto da revista:
BIOTECNOLOGIA
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Estados Unidos