High throughput single cell counting in droplet-based microfluidics.
Sci Rep
; 7(1): 1366, 2017 05 02.
Article
em En
| MEDLINE
| ID: mdl-28465615
ABSTRACT
Droplet-based microfluidics is extensively and increasingly used for high-throughput single-cell studies. However, the accuracy of the cell counting method directly impacts the robustness of such studies. We describe here a simple and precise method to accurately count a large number of adherent and non-adherent human cells as well as bacteria. Our microfluidic hemocytometer provides statistically relevant data on large populations of cells at a high-throughput, used to characterize cell encapsulation and cell viability during incubation in droplets.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Técnicas Analíticas Microfluídicas
/
Ensaios de Triagem em Larga Escala
/
Análise de Célula Única
Limite:
Humans
Idioma:
En
Revista:
Sci Rep
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
França