Human alveolar macrophage function: differences between smokers and nonsmokers.
J Leukoc Biol
; 44(5): 313-8, 1988 Nov.
Article
em En
| MEDLINE
| ID: mdl-2846725
ABSTRACT
Human alveolar macrophages and peripheral blood monocytes were obtained from smoking and nonsmoking normal volunteers. The macrophages and monocytes were incubated in vitro with bacterial lipopolysaccharide (LPS). The oxidative metabolic response of these cells was measured by superoxide anion production. Macrophages from smokers were suppressed in their superoxide anion response to LPS activation as compared to macrophages from nonsmokers. Monocytes from smokers and nonsmokers were not different. The cytotoxic properties of these macrophages and monocytes were assessed by an in vitro 3H-thymidine release assay against various allogeneic target cells. Macrophages and monocytes exposed to LPS were rendered tumoricidal. Macrophages from nonsmokers appeared to generate greater cytotoxic activity than macrophages from smokers. Macrophages from both smokers and nonsmokers were cytotoxic for three different tumorigenic cell lines but not for a nontumorigenic cell line. Monocytes from smokers and nonsmokers were not different in cytotoxic activity. We conclude that macrophages from both smokers and nonsmokers can be activated after exposure to LPS; however, macrophages from smokers may be slightly suppressed in their responses.
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Bases de dados:
MEDLINE
Assunto principal:
Alvéolos Pulmonares
/
Fumar
/
Macrófagos
Limite:
Adult
/
Humans
/
Middle aged
Idioma:
En
Revista:
J Leukoc Biol
Ano de publicação:
1988
Tipo de documento:
Article