Use of imaging software for assessment of the associations among zona pellucida thickness variation, assisted hatching, and implantation of day 3 embryos.
J Assist Reprod Genet
; 34(10): 1261-1269, 2017 Oct.
Article
em En
| MEDLINE
| ID: mdl-28685392
ABSTRACT
PURPOSE:
The aim of this study was to determine if zona pellucida thickness variation (ZPTV) is associated with implantation and if this relationship changes with use of assisted hatching (AH).METHODS:
Day 3 embryos from single or double embryo transfers (DETs) performed between 2014 and 2016 were included. ZPTV was assessed by examining photographs taken before transfer using an automated image processing platform to segment the zona pellucida (ZP) with an active contour technique. One hundred points were obtained of ZP thickness (ZPT) of each embryo to calculate ZPTV ([maximum ZPT-mean ZPT]/mean ZPT). Logistic regression was used to calculate the odds ratio (OR) and 95% confidence intervals (CI) of implantation by tertile of ZPTV. Maternal age and AH were adjusted for a priori. Other cycle and embryo characteristics were adjusted for if they altered the continuous effect estimate by >10%.RESULTS:
There was no statistically significant association between ZPTV and implantation across tertiles although embryos with greater ZPTV showed a trend of decreased implantation (Tertile 2 (T2) versus Tertile 1 (T1), OR = 0.80, CI = 0.50-1.28; Tertile 3 (T3) versus Tertile 1 (T3), OR = 0.75, CI = 0.47-1.20). While similar nonsignificant trends for the association between ZPTV and implantation were observed across tertiles after stratification of embryos hatched or not, embryos with the greatest ZPTV had slightly higher odds for implantation when AH was utilized (T3 vs. T1 with AH, OR = 0.89, CI = 0.49-1.62; without AH, OR = 0.61, 0.29-1.27).CONCLUSION:
ZPTV was not associated with implantation after day 3 transfer. This finding did not vary by use of AH.Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Implantação do Embrião
/
Zona Pelúcida
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Processamento de Imagem Assistida por Computador
/
Software
Tipo de estudo:
Observational_studies
/
Risk_factors_studies
Limite:
Adult
/
Female
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Humans
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Pregnancy
Idioma:
En
Revista:
J Assist Reprod Genet
Assunto da revista:
GENETICA
/
MEDICINA REPRODUTIVA
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Estados Unidos