Characterization of PTV-12, a newly described porcine teschovirus serotype: in vivo infection and cross-protection studies.
J Gen Virol
; 98(7): 1636-1645, 2017 Jul.
Article
em En
| MEDLINE
| ID: mdl-28691894
ABSTRACT
Porcine teschoviruses (PTVs) constitute 1 of the 31 genera within the Picornaviridae family, comprising at least 13 genetic types (PTV-1 to PTV-13), of which only 11 (PTV-1 to PTV-11) have been recognized as serotypes to date. Specific for swine and wild boars, most PTVs are usually non-pathogenic, but some viral variants cause severe disorders in the central nervous system (Teschen disease) or milder signs (Talfan disease), as well as reproductive, digestive and respiratory disorders and skin lesions. Previous studies revealed a high diversity of teschoviruses circulating in Spanish pig populations. Phylogenetic analysis performed with these sequences and others available in GenBank disclosed 13 clusters, 11 of which corresponded to the known PTV serotypes, and 1 of 2 additional groups is represented by isolate CC25, whose full-length genomic sequence has been obtained. This group is new to science, and was putatively named PTV-12. Here, a complete characterization of this isolate is presented, including the experimental infection of minipigs to assess tissue tropism and possible pathogenicity in vivo in the host species. In addition, using this experimental animal model, we investigated whether a pre-existing infection with this PTV-12 isolate could confer cross-protection against infection with a heterotypic PTV-1 virulent strain. Based on phylogenetic analysis and serological data, we propose CC25 as the prototype strain of a new teschovirus serotype, PTV-12.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Doenças dos Suínos
/
Porco Miniatura
/
Infecções por Picornaviridae
/
Teschovirus
/
Tropismo Viral
/
Proteção Cruzada
Limite:
Animals
País/Região como assunto:
Europa
Idioma:
En
Revista:
J Gen Virol
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Espanha