Biochemical and physiological responses of Gracilaria tenuistipitata uuder two different nitrogen treatments.
Physiol Plant
; 88(4): 631-637, 1993 Aug.
Article
em En
| MEDLINE
| ID: mdl-28741779
ABSTRACT
The influence of nitrogen on ribulose-1.5-bisphosphate carboxylase/oxygenase (Rubisco. EC 4.1.1.39) content is poorly understood in macroalgae. N-deficient Gracilaria tenuistipitata Zhang et Xia var. liui was cultivated in the laboratory under constant light intensity and temperature. Biochemical and physiological variables were monitored after a high (1 mM) or low (o. 1 mM) nitrate pulse. Rubisco content in crude extracts was estimated by SDS-PAGE with the Coomassie Blue Staining procedure. Nitrate was consumed immediately in the low-N treatment, but there was always an external nitrate source in the high-N treatment. Total soluble proteins and phycobiliproteins decreased as internal nitrogen diminished in the low-N treatment, but kept fairly constant in N-sufficient conditions. However, Rubisco content increased until the 7th day and then started to decrease in both cases. Fresh weight increment showed a better correlation with Rubisco than with pigment content.
Texto completo:
1
Bases de dados:
MEDLINE
Idioma:
En
Revista:
Physiol Plant
Ano de publicação:
1993
Tipo de documento:
Article
País de afiliação:
Espanha