MicroRNA-150 controls differentiation of intraepithelial lymphocytes through TGF-ß receptor II regulation.
J Allergy Clin Immunol
; 141(4): 1382-1394.e14, 2018 04.
Article
em En
| MEDLINE
| ID: mdl-28797734
ABSTRACT
BACKGROUND:
Intraepithelial lymphocytes (IELs) in the intestines play pivotal roles in maintaining the integrity of the mucosa, regulating immune cells, and protecting against pathogenic invasion. Although several extrinsic factors, such as TGF-ß, have been identified to contribute to IEL generation, intrinsic regulatory factors have not been determined fully.OBJECTIVE:
Here we investigated the regulation of IEL differentiation and the underlying mechanisms in mice.METHODS:
We analyzed IELs and the expression of molecules associated with IEL differentiation in wild-type control and microRNA (miRNA)-150 knockout mice. Methotrexate was administered to mice lacking miR-150 and control mice.RESULTS:
miR-150 deficiency reduced the IEL population in the small intestine and increased susceptibility to methotrexate-induced mucositis. Evaluation of expression of IEL differentiation-associated molecules showed that miR-150-deficient IELs exhibited decreased expression of TGF-ß receptor (TGF-ßR) II, CD103, CD8αα, and Runt-related transcription factor 3 in all the IEL subpopulations. The reduced expression of TGF-ßRII in miR-150-deficient IELs was caused by increased expression of c-Myb/miR-20a. Restoration of miR-150 or inhibition of miR-20a recovered the TGF-ßRII expression.CONCLUSION:
miR-150 is an intrinsic regulator of IEL differentiation through TGF-ßRII regulation. miR-150-mediated IEL generation is crucial for maintaining intestinal integrity against anticancer drug-induced mucositis.Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Diferenciação Celular
/
MicroRNAs
/
Linfócitos Intraepiteliais
/
Receptor do Fator de Crescimento Transformador beta Tipo II
/
Mucosa Intestinal
/
Intestino Delgado
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
J Allergy Clin Immunol
Ano de publicação:
2018
Tipo de documento:
Article