Role of NADPH oxidase in radiation-induced pro-oxidative and pro-inflammatory pathways in mouse brain.
Int J Radiat Biol
; 93(11): 1257-1266, 2017 11.
Article
em En
| MEDLINE
| ID: mdl-28880721
ABSTRACT
PURPOSE:
The present study was designed to investigate our hypothesis that NADPH oxidase plays a role in radiation-induced pro-oxidative and pro-inflammatory environments in the brain. MATERIALS ANDMETHODS:
C57BL/6 mice received either fractionated whole brain irradiation or sham-irradiation. The mRNA expression levels of pro-inflammatory mediators, such as TNF-α and MCP-1, were determined by quantitative real-time RT-PCR. The protein expression levels of TNF-α, MCP-1, NOX-2 and Iba1 were detected by immunofluorescence staining. The levels of ROS were visualized by in situ DHE fluorescence staining.RESULTS:
A significant up-regulation of mRNA and protein expression levels of TNF-α and MCP-1 was observed in irradiated mouse brains. Additionally, immunofluorescence staining of Iba1 showed a marked increase of microglial activation in mouse brain after irradiation. Moreover, in situ DHE fluorescence staining revealed that fractionated whole brain irradiation significantly increased production of ROS. Furthermore, a significant increase in immunoreactivity of NOX-2 was detected in mouse brain after irradiation. On the contrary, an enhanced ROS generation in mouse brain after irradiation was markedly attenuated in the presence of NOX inhibitors or NOX-2 neutralizing antibody.CONCLUSIONS:
These results suggest that NOX-2 may play a role in fractionated whole brain irradiation-induced pro-oxidative and pro-inflammatory pathways in mouse brain.Palavras-chave
Texto completo:
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Bases de dados:
MEDLINE
Assunto principal:
Encéfalo
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Glicoproteínas de Membrana
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NADPH Oxidases
Limite:
Animals
Idioma:
En
Revista:
Int J Radiat Biol
Assunto da revista:
RADIOLOGIA
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Estados Unidos