Tissue cultivation as a method for preservation of human foetal islet parenchyma--a correlated biochemical, immunohistochemical and morphometric investigation.
Diabetes Res
; 5(4): 157-61, 1987 Aug.
Article
em En
| MEDLINE
| ID: mdl-2889556
ABSTRACT
Tissue culture of human foetal pancreas slices, obtained at a gestational age between 10 and 19 weeks, as a method of preservation and to pool the material before transplantation was investigated. Before and after 2 weeks of culture the pancreatic insulin content, the insulin secretion in response to glucose and isobutylmethylxanthin (IBMX) as well as the protein biosynthesis were measured. In addition, the distribution of the insulin immunoreactive cells was examined, as well as their relative volume density. After 2 weeks' culture an increase of the basal insulin secretion was observed; this was probably due to the glucose content (10 mmol/l) in the culture medium. Neither the stimulated insulin secretion, nor the protein biosynthesis, the insulin content, and the B-cell volume were altered by the used culture conditions. It was concluded that tissue culture is a suitable method to preserve human foetal pancreas slices before transplantation.
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Bases de dados:
MEDLINE
Assunto principal:
Ilhotas Pancreáticas
Limite:
Humans
Idioma:
En
Revista:
Diabetes Res
Assunto da revista:
ENDOCRINOLOGIA
Ano de publicação:
1987
Tipo de documento:
Article