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Novel KIR genotypes and gene copy number variations in northeastern Thais.
Chaisri, Suwit; Traherne, James A; Jayaraman, Jyothi; Romphruk, Amornrat; Trowsdale, John; Leelayuwat, Chanvit.
Afiliação
  • Chaisri S; Chulabhorn International College of Medicine (CICM), Thammasat University, Klong Luang, Thailand.
  • Traherne JA; The Centre for Research and Development of Medical Diagnostic Laboratories (CMDL), Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.
  • Jayaraman J; Division of Immunology, Department of Pathology, University of Cambridge, Cambridge, UK.
  • Romphruk A; Division of Immunology, Department of Pathology, University of Cambridge, Cambridge, UK.
  • Trowsdale J; The Centre for Research and Development of Medical Diagnostic Laboratories (CMDL), Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.
  • Leelayuwat C; Blood Transfusion Centre, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Immunology ; 153(3): 380-386, 2018 03.
Article em En | MEDLINE | ID: mdl-28950036
KIR (Killer Immunoglobulin-like Receptor) variants influence immune responses and are genetic factors in disease susceptibility. Using sequence-specific priming PCR, we have previously described the diversity of KIR genes in term of presence/absence in northeastern Thais (NETs). To provide additional resolution beyond conventional methods, quantitative PCR was applied to determine KIR copy number profiles. Novel expanded and contracted KIR copy number profiles were identified at cumulatively high frequencies. These all comprise haplotypes with duplication (6·9%) or deletion (2·7%) of KIR3DL1/S1 along with adjacent genes. Five expanded KIR profiles comprised haplotypes with duplications of KIR2DP1, 2DL1, 3DP1, 2DL4, 3DL1/S1 and 2DS1/4, whereas two contracted profiles contained only a single copy of KIR3DP1, 3DL1/S1 and 2DL4. Using a KIR haplotype prediction program (KIR Haplotype Identifier), 14% of NET haplotypes carried atypical haplotypes based on the gene copy number data.
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Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Haplótipos / Receptores KIR / Variações do Número de Cópias de DNA Tipo de estudo: Prognostic_studies Limite: Humans País/Região como assunto: Asia Idioma: En Revista: Immunology Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Tailândia

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Haplótipos / Receptores KIR / Variações do Número de Cópias de DNA Tipo de estudo: Prognostic_studies Limite: Humans País/Região como assunto: Asia Idioma: En Revista: Immunology Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Tailândia