Novel KIR genotypes and gene copy number variations in northeastern Thais.
Immunology
; 153(3): 380-386, 2018 03.
Article
em En
| MEDLINE
| ID: mdl-28950036
KIR (Killer Immunoglobulin-like Receptor) variants influence immune responses and are genetic factors in disease susceptibility. Using sequence-specific priming PCR, we have previously described the diversity of KIR genes in term of presence/absence in northeastern Thais (NETs). To provide additional resolution beyond conventional methods, quantitative PCR was applied to determine KIR copy number profiles. Novel expanded and contracted KIR copy number profiles were identified at cumulatively high frequencies. These all comprise haplotypes with duplication (6·9%) or deletion (2·7%) of KIR3DL1/S1 along with adjacent genes. Five expanded KIR profiles comprised haplotypes with duplications of KIR2DP1, 2DL1, 3DP1, 2DL4, 3DL1/S1 and 2DS1/4, whereas two contracted profiles contained only a single copy of KIR3DP1, 3DL1/S1 and 2DL4. Using a KIR haplotype prediction program (KIR Haplotype Identifier), 14% of NET haplotypes carried atypical haplotypes based on the gene copy number data.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Haplótipos
/
Receptores KIR
/
Variações do Número de Cópias de DNA
Tipo de estudo:
Prognostic_studies
Limite:
Humans
País/Região como assunto:
Asia
Idioma:
En
Revista:
Immunology
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Tailândia