Physicochemical properties and anticoagulant activity of polyphenols derived from Lachnum singerianum.
J Food Drug Anal
; 25(4): 837-844, 2017 Oct.
Article
em En
| MEDLINE
| ID: mdl-28987360
ABSTRACT
In this study, polyphenols (LSP) were obtained from the fermentation broth of Lachnum singerianum. Two fractions were isolated by Sephadex LH-20 chromatographic column, and the primary fraction (LSP-1) was collected. The comprehensive physicochemical properties of phenolic acids and polyhydroxy phenolic compounds of LSP-1 were determined by UV-visible spectroscopy, Fourier transform infrared spectroscopy, and gas chromatography-mass spectrometry. Results of anticoagulant activity assay in vitro showed that LSP-1 could lengthen prothrombin time, activated partial thromboplastin time, and thrombin time of mouse plasma. In addition, anticoagulant activity results in vivo showed that high dose of LSP-1 could significantly prolong bleeding time, coagulation time, prothrombin time, activated partial thromboplastin time, and thrombin time of hypercoagulable mice induced by adrenaline, reduce the content of fibrinogen and enhance antithrombin III activity. All results indicated that the LSP-1 could serve well as an anticoagulant, and might be used as a potential natural drug candidate for thrombosis.
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Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Ascomicetos
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Trombose
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Polifenóis
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Anticoagulantes
Limite:
Animals
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Humans
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Male
Idioma:
En
Revista:
J Food Drug Anal
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
China